Effect of oxidised repeseed oil with garlic on the concentration of 7-ketocholesterol, malondialdehyde, and free fatty acids in hypercholesterolaemic rabbits

• Autorzy: Zalejska-Fiolka J , Kasperczyk A. , Kasperczyk S. , Blaszczyk U. , Birkner E.
• Języki publikacji: EN

Abstrakty

EN

The influence of a balanced diet (21 g% protein, 34 g% fat, 45 g% carbohydrate) with an isocaloric addition of non-oxidised or oxidised rapeseed oil, with and without garlic, on the development of hypercholesterolaemia was examined in 18 adult male rabbits divided into 3 equal groups. The rabbits from group 1 were fed fresh rapeseed oil, group II - received oxidised rapeseed oil, and group III - was given oxidised rapeseed oil and garlic. The concentration of 7-ketocholesterol and malondialdehyde (MDA) in plasma and free fatty acids (FAME) in blood serum was determined. The experiment lasted 24 weeks. At the beginning, and every six weeks, the rabbits were weighed and blood was taken. After the experiment was completed, the aorta was dissected for histological examinations. It was found that oxidised rapeseed oil caused an increase in the concentration of 7-ketocholesterol and FAME at the end of the experiment. MDA concentration increased in the 6th week of the experiment but did not appreciably change at the end of it. The obtained results suggested that the diet caused the development of hypercholesterolaemic alterations in the aorta wall and increased temporarily the level of 7-ketocholesterol, FAME, and MDA. Diet rich in oxidised oil modified significantly homeostasis of lipids in plasma and blood serum. The administration of garlic in such a diet inhibited atherosclerotic changes in the aorta wall and this seemed to be related to the decreasing concentration of 7-ketocholesterol and MDA in plasma and FAME in blood serum.

Słowa kluczowe

EN

determination; garlic; oxidation; blood serum; 7-ketocholesterol; malondialdehyde; hypercholesterolemia; rabbit; free fatty acid; rapeseed oil

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2007
• Tom: 51
• Numer: 3
• Opis fizyczny: p.431-438,fig.,ref.

Twórcy

autor

Zalejska-Fiolka J

• Silesian Medical University, 40-006 Katowice, Poland

autor

Kasperczyk A.

autor

Kasperczyk S.

autor

Blaszczyk U.

autor

Birkner E.

Bibliografia

• 1. Bhadra S., Arshad M.A., Rymaszewski Z., Norman E., Wherley R., Subbiah T.: Oxidation of cholesterol moiety of LDL in the presence of endothelial cells and Cu⁺⁺ ions: identification of major products and their effects. Biochem Biophys Res Commun 1991, 176, 431-440.
• 2. Birkner E., Kasperczyk S., Kasperczyk A., Zalejska- Fiolka J., Zwirska-Korczala K., Stawiarska-Pieta B., Grucka-Mamczar E.: Metabolic and antioxidative changes in liver steatosis induced by high-fat, low-carbohydrate diet in rabbits. J Physiol Pharmacol 2005, 56, 45-58.
• 3. Blankenship J.W., Van Gent C.M., Sandberg L.B., Roos P.J., Scharffenberg J.A.: Oxysterol incorporation into rat aorta resulting in elastin compositional changes. Lipids 1991, 26, 381-384.
• 4. Durak I., Ozturk H.S., Olcay E., Can B., Kavutcu M.: Effects of garlic extract on oxidant/antioxidant status and atherosclerotic plaque formation in rabbit aorta. Nutr Metab Cardiovasc Dis 2002, 12, 141-147.
• 5. Dzeletovic S., Breuer O., Lund E., Diczfalusy U.: Determination of cholesterol oxidation products in human plasma by isotope dilution-mass spectrometry. Anal Biochem 1995, 22, 73-80.
• 6. Hodis H.N., Chauhan A., Hashimoto S., Crawford D.W., Sevanian A.: Probucol reduces plasma and aortic wall oxysterol levels in cholesterol fed rabbits independently of its plasma cholesterol lowering effect. Atherosclerosis 1992, 96, 125-134.
• 7. Hodis H.N., Kramsch D.M., Avogaro P., Bittolo-Bon G., Cazzolato G., Hwang J., Peterson H., Sevanian A.: Biochemical and cytotoxic characteristics of an in vivo circulating oxidized low density lipoprotein (LDL-). J Lipid Res 1994, 35, 669-677.
• 8. Kim K.M., Chun S.B., Koo M.S., Choi W.J., Kim T.W., Kwon Y.G., Chung H.T., Billiar T.R., Kim Y.M.: Differential regulation of NO availability from macrophages and endothelial cells by the garlic component S-allyl cysteine. Free Radic Biol Med 2001, 30, 747-756.
• 9. Lyons M.A., Samman S., Gatto L., Brown A.: Rapid hepatic metabolism of 7-ketocholesterol in vivo: implications for dietary oxysterols. J Lipid Res 1999, 40, 1846-1857.
• 10. Mahfouz M.M., Kummerow F.A.: Cholesterol-rich diets have different effects on lipid peroxidation, cholesterol oxides, and antioxidant enzymes in rats and rabbits. J Nutr Biochem 2000, 11, 293-302.
• 11. Maor I., Mandel H., Aviram M.: Macrophage uptake of oxidized LDL inhibits lysosomal sphingomyelinase, thus causing the accumulation of unesterified cholesterol- sphingomyelin-rich particles in the lysosomes. A possible role for 7-ketocholesterol. Arterioscler Thromb Vase Biol 1995, 15, 1378-1387.
• 12. Mol J., Rostański K., Szulik J.: The Oenothera species evaluation by gas chromatography. Riv Ital Sostanze Grasse 2001, 1, 35-40.
• 13. Oka K., Yasuhara M., Suzumura K., Tanaka K., Sawamura T.: Antioxidants suppress plasma levels of lectin-like oxidized low-density lipoprotein receptor-ligands and reduce atherosclerosis in watanabe heritable hyperlipidemic rabbits. J Cardiovasc Pharmacol 2006, 48, 177-183.
• 14. Polish Standardizing Committee - Animal and vegetable oils and fats - determination of peroxide value, Norm. Alfa - Wero, Warsaw, 1996, pp. 3-7.
• 15. Polish Standardizing Committee - Animal and vegetable oils and fats - determination of iodine value, 1998, Warsaw, Norm. Alfa - Wero, pp. 3-7.
• 16. Salonen J.T.: Epidemiological studies on LDL oxidation, pro- and antioxidants and atherosclerosis. In: Free Radicals, Lipoprotein Oxidation and Atherosclerosis. Ed. Bellomo G., Finardi G., Maggi E., Rice-Evans C., Richelieu Press, London, 1995, pp. 27-52.
• 17. Schwenke D.C., Behr S.R.: Alpha-tocopherol and probucol reduce autoantibody titer to MDA-LDL in hypercholesterolemic rabbits. Free Radic Biol Med 2001, 31, 778-789.
• 18. Sevanian A., Seraglia R., Traldi P., Rossato P., Ursini F., Hodis H.: Analysis of plasma cholesterol oxidation products using gas- and high-performance liquid chromatography/mass spectrometry. Free Radic Biol Med 1994, 17, 397-409.
• 19. Smith L.L.: Cholesterol Autooxidation. New York, NY Plenum Publishing Corp., 1981.
• 20. Song S.H., Min B.I., Lee J.H., Cho K.S.: Chronological effects of atherogenic diets on the aorta, liver and spleen of rabbits. J Korean Med 2000, 15, 413-419.
• 21. Steinberg D., Parthasarathy S., Carew T.E., Khoo J.C., Witztum J.L.: Beyond cholesterol: modifications of low- density lipoprotein that increase its atherogenicity. N Engl J Med 1989, 320, 915-924.
• 22. Tomasik A., Poloński L., Wojciechowska C., Fiolka J., Skrzep-Poloczek B., Tarnawski R., Wodniecki J., Jacheć W.: Effect of four week long treatment with fenofibrate on the level of 7-ketocholesterol in hyperlipidemic patients. Cardiovasc Risk Factor 2001, 8, 174-179.
• 23. Tsimikas S., Aikawa M., Miller F.J. Jr, Miller E.R., Torzewski M., Lentz S.R., Bergmark C., Heistad D.D., Libby P., Witztum J.L.: Increased plasma oxidized phospholipid:apolipoprotein B-100 ratio with concomitant depletion of oxidized phospholipids from atherosclerotic lesions after dietary lipid-lowering: a potential biomarker of early atherosclerosis regression. Arterioscler Thromb Vase Biol 2007, 27, 175-181.
• 24. Verbeuren T.J.: Experimental models of thrombosis and atherosclerosis. Therapie 2006, 61, 379-387.
• 25. Witztum J.L.: The oxidation hypothesis of atherosclerosis. Lancet 1994, 344, 793-795.
• 26. Zalejska-Fiolka J., Kasperczyk S., Kasperczyk A., Birkner E., Grucka-Mamczar E., Stawiarska-Pięta B., Schneider A.: The influence of oxidant vegetable oil and garlic extract upon the development of experimental atherosclerosis in rabbits. Bull Vet Inst Pulawy 2004, 48, 453-459.
• 27. Zalejska-Fiolka J.: The influence of garlic extract on oxidation of edible oils. Riv Ital Sostanze Grasse 2001, 6, 343-346.
• 28. Zalejska-Fiolka J.: The influence of garlic extract on the oxidation process of edible oils. Part II. Riv Ital Sostanze Grasse 2002, 10, 365-368.
• 29. Zawistowski S.: Technika histologiczna, histologia oraz podstawy histopatologii. Edited by PZWL, Warsaw, 1986, pp. 108-122.