PL
W przypadku podejrzenia bakteriemii, jednym z podstawowych badań są posiewy krwi. Celem naszych badań była analiza mikrobiologiczna 11170 próbek krwi pobranych od chorych hospitalizowanych w Wojewódzkim Szpitalu Specjalistycznym im. L. Rydygiera w Krakowie w okresie od 1997 do 2000 roku. Odsetek wyników dodatnich, odpowiadających wykryciu drobnoustrojów o znaczeniu klinicznym wynosił 16,9%.
EN
The aim of our investigations was the microbiological analysis together with the evaluation of sensitivity of bacteria frequently isolated from blood cultures. Blood samples were taken from patients with symptoms suggesting bacteremia in Rydygier's Hospital in Cracow. A total of 11170 blood samples taken from 1997 to 2000 were tested. Automatic VITAL system (bioMerieux) was applied to culture and detect microorganisms. Bacteria were identified by ATB system (bioMerieux). Susceptibility was detected by ATB and disc diffusion method. Percentage of positive results relating to detection of microorganisms of clinical significance was 116.9 % (1891 cultures). Staphylococcus spp. (Staph. epidermidis in range 22.8% to 21.9%), Enterococcus spp. and Streptococcus spp. were most frequently isolated species among aerobic Gram-positive bacteria. In 2000, compared to 1997 the number of isolates of MRSA increased considerably (from 1.8% to 6.8%). In blood infections the increase of frequency of E. coli bacteria was also noted: 6.1% and 11.4% in 1997 and 2000, respectively. Among non-fermentant bacilli the percentage of occurrence of P. aeruginosa in the period of 4 years was comparable in the range 7.3% in 1997 to 7.2% in 2000. The increase in the frequency of blood infections of A. baumanii was also noticed (respectively from 4,8% to 9.9%). Susceptibility of P. aeruginosa strains to selected ß-lactame antibiotics and aminoglycosydes increased in 2000 in comparison to 1999. A. baumanii strains were 100% sensitive only to imipenem.