Design based stereological methods for estimating the total number of objects in histological material

• Autorzy: West M J
• Języki publikacji: EN

Abstrakty

EN

The principle that formed the basis of the most popular “assumption based” stereological methods for counting cells that were available prior to the advent of the more recently developed “design based” methods will be described in general terms. The major weaknesses inherent in the older methods will be described, along with how they have been eliminated by the design based methods.

Słowa kluczowe

EN

cell counting; histological material; anatomy; stereological method; stereology

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2001
• Tom: 60
• Numer: 1
• Opis fizyczny: p.11-19,fig.

Twórcy

autor

West M J

• University of Aarhus, 8000 Aarhus C, Denmark

Bibliografia

• 1. Bendtsen TF, Nyengaard JR (1998) Unbiased estimation of particle number using sections — An historical perspective with special reference to the stereology of glomeruli. J Microsc, 153: 93–102.
• 2. Cruz-Orive LM (1997) Stereology of single objects. J Microsc, 186: 93–107.
• 3. Cruz-Orive L, Weibel ER (1990) Recent stereological methods for cell biology: a brief survey. Am J Physiol, 258 (Lung Cell Mol Physiol 2): L148–L156.
• 4. Geinisman Y, Gundersen HJG, West MJ (1996) Unbiased stereological estimation of the total number of synapses in a brain region. J Neurocytology 25: 805–819.
• 5. Gokhale AM, Drury WJ (1994) Efficient measurement of microstructural surface area using trisector. Metallurgical and materials transactions. 25A: 919–928.
• 6. Gundersen HJG (1977) Notes on the estimation of the numerical density of arbitrary particles: the edge effect. J Microsc, 111: 219–233.
• 7. Gundersen HJG (1986) Stereology of arbitrary particles. A review of unbiased number and size estimators and the presentation of some new ones, in memory of William R. Thompsen. J Microsc, 143: 3–45.
• 8. Gundersen HJG, et al. (1988a) Some new, simple, and efficient stereological methods and their use in pathological research and diagnosis. APMIS, 96: 379–394.
• 9. Gundersen HJG, et al. (1988b) The new stereological tools: Disector, fractionator, nucleator, and point sampled intercepts and their use in pathological research and diagnosis. APMIS, 96: 857–881.
• 10. Hyman BT, Gomez-Isla T, Irizarry MC (1998) Stereology: a practical primer for neuropathology. J Neuropathol Exp Neurol, 57: 305–310.
• 11. Jensen EBV, Gundersen HJG (1993) The rotator. J Microsc, 170: 35–44.
• 12. Konigsmark BW (1970) Methods for the counting of neurons. In: Nauta WJH, Ebbesson SOE (eds.). Contempory Research Methods in Neuroanatomy. Springer-Verlag, New York, pp. 315–340.
• 13. Larsen JO (1998) Global spatial sampling with isotropic virtual planes: estimators of length density and total length in thick, arbitrarily oriented sections. J Microsc, 191: 238–248.
• 14. Mouton PM, Gokhale AM, Ward NL, West MJ (2001) Unbiased Length Estimation Using Isotropic Spherical Probes. J Microsc, (under revision).
• 15. Sterio DC (1984) The unbiased estimation of number and size of arbitrary particles using the disector. J Microsc, 134: 127–136.
• 16. West MJ (1993) New stereological methods for counting neurons. Neurobiol Aging, 14: 275–285.
• 17. West MJ (1999) Stereological Methods for Estimating the Total Number of Neurons and Synapses: Issues of Precision and Bias. Trends Neurosci, 22: 51–61.
• 18. West MJ, Gundersen HJG (1990) Unbiased stereological estimation of the number of neurons in the human hippocampus. J Comp Neurol, 296: 1–22.
• 19. West MJ, Slomianka L, Gundersen HJG (1991) Unbiased stereological estimation of the total number of neurons in the subdivisions of the rat hippocampus using the optical fractionator. Anat Rec, 231: 482–497.
• 20. West, MJ, Østergaard K, Andreassen O, Finsen B (1996) Estimation of the number of somatostatin neurons in the striatum: An in situ study using the optical fractionator. J Comp Neurol, 370: 11–22.