The study aimed at determining which part of magnesium permanently bound with bakers yeasts S. cerevisiae No. 102 during batch culture in control (YPD) and experimental medium (YPD with the addition of magnesium) remains in the cell wall and which undergoes intracellular bio-accumulation. The experimental media were supplemented with the amount of MgCl2*6H2O providing 1.25 g/dm3 content of Mg2+ ions. The cultures were run for 24 h at 28°C in a reciprocating shaker which provided aerobic conditions of the process. The application of enzymatic protoplastisation and mechanical disintegration of the yeast cells (at a temperature of liquid nitrogen) enabled determination of the contents of magnesium and total protein in both cell walls and protoplasts.
Birch R. M., Walker G. M., 2000. Influence of magnesium ions on heat shock into S. cerevisiae cell by hypotonic downshift. Enzyme Microbiol. Technol. 26, 678-687.
Błażejak S., Duszkiewicz-Reinhard W., Gniewosz M., Rostkowska-Demner E., Domurad E.,2002. The study of Saccharomyces cerevisiae brewery yeast strain capacity of binding with magnesium in dynamic conditions. EJPAU Food Sci. Technol. 1(2).
Bui M. D., Gregan J., Jarosch E., Ragnini A., Schweyen R., J., 1999. The bacterial magnesium transporter CorA can functionally substitute for its putative homologue Mrs2p in the yeast inner mitochondrial membrane. J. Biol. Chem. 274, (29), 20438-20443.
Burbianka M., Pliszka A., 1983. Mikrobiologia żywności [Food microbiology]. PZWL Warsaw [in Polish].
Chmiel A., 1998. Biotechnologia. Podstawy mikrobiologiczne i biochemiczne [Biotechnology. Microbiology and biochemistry fundamental] PWN, Warsaw [in Polish].
Ciesarova Z., Smogrovicova D., Domeny Z., 1996. Enhancement of yeast ethanol tolerance by calcium and magnesium. Fol. Microbiol. 41 (6 ), 485-488
Duszkiewicz-Reinhard W., Gniewosz M., Błażejak St., Bańkowski A., 2002. Badanie zdolności wiązania magnezu przez drożdże piekarskie Saccharomyces cerevisiae w warunkach hodowli stacjonarnej [The study of Saccharomyces cerevisiae brewery yeast strain capacity of binding with magnesium in stationary conditions]. Acta Sci. Pol. Technol. Alim. 1(1), 17-26 [in Polish].
Emelyanowa E. V., 2001. Relationship between magnesium and iron uptake by the yeast Candida ethanolica. Proc. Biochem. 36, 517-523.
Graschopf A., Schtadler J. A., Hoellerer M., K., Eder S., Sieghardt M., 2001. The yeast plasma membrane protein Alr controls Mg2+ homeostasis and is subject to Mg2+ dependent control of its synthesis and degradation. J. Biol. Chem. 19 (276), 16216-16222.
Klepacka M., 2000. Analiza żywności, skrypt do ćwiczeńm [Food analysis]. Wyd. SGGW, Warsaw [in Polish].
Kurtzman C. P., Fell J. W., 1998. The yeast taxonomic study. Elsevier Sci., Amsterdam.
Lipke P. N., Ovalle R., 1998. Cell wall architecture in yeast: new structure and new challanges. J. Bacteriol. 180 (15), 3735-3740.
Lyticase L2524. 2002. Materiały firmy Sigma-Aldrich [Sigma Aldrich handbook]
MacDiarmid C. W., Gardner R. C., 1998. Overexpresion of the Saccharomyces cerevisiae magnesium transport system confers resistance to aluminium ion. J. Biol. Chem. 273 (3), 1727-1732.
Mardarowicz L., 1997. Drożdże w żywieniu drobiu [The yeast in poultry nourishment]. Pol. Drob. 9, 14-16 [in Polish].
Muller E., Loeffler W., 1987. Zarys mikologii dla przyrodników i lekarzy [Outline of micology for natural scientist and doctor]. PWRiL, Warsaw [in Polish].
Pasternakiewicz A., Tuszyński T., 1997. Effects of calcium, magnesium, cobalt (III) and zinc cations on the Saccharomyces cerevisiae growth. Pol. J. Food Nutr. Sci. 47 (4), 61-70.
Robinson R. K., Batt C. A.,Patel P. D., 2000. Encyclopedia of Food Microbiology. Academic Press, Oxford.
Sikorski Z., 2000. Chemia żywności: skład, przemiany i właściwości żywności [Food chemistry: composition, changes and properties of food]. WNT, Warsaw [in Polish].
Soral-Śmietana M., Wronkowska M., Świgoń A., Kłębukowska L., 1999. Biopierwiastki - możliwości tworzenia kompleksów z polimerami organicznymi [Biochemical elements- possibility to create of complex with organic polymers]. Biul. Magnezol. 4 (2), 418-423.
Suizu T. Tsutsumi H., Kawado A., Imayasu S., Inose T., Kimura A., Murata K., 1994. Induction of yeast sporulation by lysine related compounds and glutathione rich conditions. J. Ferm. Bioeng. 77 (5), 568572.
Tuszyński T., Pasternakiewicz A., 2000. Bioaccumulation of metal ions by yeast cell of Saccharomyces cerevisiae. Pol. J. Food Nutr. Sci. 4, 3139.
Vandergrift B., 1991. Bioplexes: Practical application. Biotechnology in the feed industry. Proc. Alltechs Seventh Annual Symp.
Walker G. M., 1994. The roles of magnesium in biotechnology. Crit. Rev. Biotechnol. 14 (4), 311-354.