EN
Tissue cultures were derived from the roots and above-ground part of sterile seedlings of Leuzea carthamoicles DC. Testing of the growth on the medium according to Murashige and Skoog with the addition of various combinations and concentrations of growth substances proved that at calluses derived from the aerial part the highest stimulative effect was carried out by the combination of 2,4-D (1.0 mg1-1) and IBA (0.5 mg1-1). The average value of the increment was 544%. Callus cultures derived from the roots grew less intensively than the cultures derived from the aerial parts. The highest stimulative effect was found at 2,4-D (1.0 mg1-1) in combination with BA (1.0 mg1-1) - 230%. During experiments for a certain level of morphological differentiation bud-formation was reached and after rooting plant regeneration. When substituting 1AA by Meristimul and its combination with kinetin as well, deformed buds were formed. Orientation chromatographic analysis of callus extracts and leaves of regenerated plants has not proved the presence of ecdysteroids so far.
PL
Hodowle tkankowe Leuzea carthamoides DC. otrzymywano z korzeni oraz nadziemnych części jałowych sadzonek tej rośliny. Najlepiej hodowle te rozwijały się na podłożu Murashige i Skooga wzbogaconym o 2,4-D (1,0 mg1-1) i IBA (0,5 mg1-1). Wzrost kalusa na tym podłożu wynosił średnio 544%. Hodowle otrzymane z nadziemnych części roślin rozwijały się bardziej intensywnie w porównaniu do kalusów otrzymanych z korzeni. Analiza chromatograficzna wyciągów z kalusa nie wykazała obecności ekdysteroidów, substancji adaptogennych występujących w sadzonkach.