UV- and MMS-induced mutagenesis of lambdaO[am]8 phage under nonpermissive conditions for phage DNA replication

• Autorzy: Krwawicz J. , Czajkowska A. , Felczak M. , Pietrzykowska I.
• Języki publikacji: EN

Abstrakty

EN

Mutagenesis in Escherichia coli, a subject of many years of study is considered to be related to DNA replication. DNA lesions nonrepaired by the error-free nucleotide excision repair (NER), base excision repair (BER) and recombination repair (RR), stop replication at the fork. Reinitiation needs translesion synthesis (TLS) by DNA polymerase V (UmuC), which in the presence of accessory proteins, UmuD', RecA and ssDNA-binding protein (SSB), has an ability to bypass the lesion with high mutagenicity. This enables reinitiation and extension of DNA replication by DNA polymerase III (Pol III). We studied UV- and MMS-induced mutagenesis of λO(am)8 phage in E. coli 594 sup host, unable to replicate the phage DNA, as a possible model for mutagenesis induced in nondividing cells (e.g. somatic cells). We show that in E. coli 594 sup cells UV- and MMS-induced mutagenesis of λO(am)8 phage may occur. This mutagenic process requires both the UmuD' and C proteins, albeit a high level of UmuD' and low level of UmuC seem to be necessary and sufficient. We com­pared UV-induced mutagenesis of λO(am)8 in nonpermissive (594 sup ) and permis­sive (C600 supE) conditions for phage DNA replication. It appeared that while the mutagenesis of XO(am)8 in 594 sup+ requires the UmuD' and C proteins, which can not be replaced by other SOS-inducible protein(s), in C600 supE their functions may be replaced by other inducible protein(s), possibly DNA polymerase IV (DinB). Muta­tions induced under nonpermissive conditions for phage DNA replication are resis­tant to mismatch repair (MMR), while among those induced under permissive condi­tions, only about 40% are resistant.

Słowa kluczowe

EN

bacteriophage; K-12 strain; DNA replication; plasmid; mutagenesis; Escherichia coli

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2003
• Tom: 50
• Numer: 4
• Opis fizyczny: p.921-939,fig.,ref.

Twórcy

autor

Krwawicz J.

• Polish Academy of Sciences, A.Pawinskiego 5A, 02-106 Warsaw, Poland

autor

Czajkowska A.

autor

Felczak M.

autor

Pietrzykowska I.

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