Induction of DNA breakage in X-irradiated nucleoids selectively stripped of nuclear proteins in two mouse lymphoma cell lines differing in radiosensitivity

• Autorzy: Kruszewski M. , Iwanenko T.
• Języki publikacji: EN

Abstrakty

EN

The role of nuclear proteins in protection of DNA against ionizing radiation and their contribution to the radiation sensitivity was examined by an alkaline version of comet assay in two L5178Y (LY) mouse lymphoma cell lines differing in sensitivity to ionizing radiation. LY-S cells are twice more sensitive to ionizing radiation than LY-R cells (D0 values of survival curves are 0.5 Gy and 1 Gy, respectively). Sequential removal of nuclear proteins by extraction with NaCl of different concentrations increased the X-ray induced DNA damage in LY-R nucleoids. In contrast, in the radiation sensitive LY-S cell line, depletion of nuclear proteins practically did not affect DNA damage. Although there is no doubt that the main cause of LY-S cells' sensitivity to ionizing radiation is a defect in the repair of double-strand breaks, our data support the concept that nuclear matrix organisation may contribute to the cellular susceptibility to DNA damaging agents.

Słowa kluczowe

EN

lymphoma; ionizing radiation; radiation sensitivity; DNA breakage; nuclear protein; mice; radiosensitivity; X-irradiated nucleoid; cell line; mouse; eukaryotic cell; DNA damage; nucleus

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1998
• Tom: 45
• Numer: 3
• Opis fizyczny: p.701-704,fig.

Twórcy

autor

Kruszewski M.

• Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warsaw, Poland

autor

Iwanenko T.

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