Age-related profile of beta-N-acetylhexosaminidase glycosylation in rat liver

• Autorzy: Litynska A. , Przybylo M.
• Języki publikacji: EN

Abstrakty

EN

β-N-acetylhexosaminidase was prepared from a liver lysosomal fraction obtained from rats between 18 days of gestation (group I) and 72 weeks of age (groups II-VI). A glycan chain analysis was performed after an electrophoresis and blotting, followed by a very sensitive detection system with highly specific digoxigenin-labelled lectins. The presence of high-mannose /hybrid type glycans, as well as their fucosylated forms was shown in all the experimental groups. Complex-type glycans with terminal sialic acid or galactose were present in all the groups except for 1-week-old rats in which only a positive reaction with lectins from Galanthus nivalis and Aleuria aurantia - was observed. Thus it may be assumed that age-related changes in the glycosylation pattern occur on the first days after birth.

Słowa kluczowe

EN

lectin; Aleuria aurantia; glycosylation; beta-N-acetylhexosaminidase; Galanthus nivalis; liver; rat

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1998
• Tom: 45
• Numer: 3
• Opis fizyczny: p.791-797,fig.

Twórcy

autor

Litynska A.

• Jagiellonian University, R.Ingardena 6, 30-060 Krakow, Poland

autor

Przybylo M.

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