Analiza mozliwosci rozprzestrzeniania sie opornosci na aminoglikozydy wsrod gronkowcow i enterokokow na podstawie kompozycji genow kodujacych AME

• Autorzy: Jarzembowski T.
• Języki publikacji: PL

Abstrakty

PL

Przeprowadzono identyfikację 6 genów kodiyących enzymy modyfikujące aminoglikozydy wśród szczepów E. faecalis, S. epidermidis, S. aureus, S. haemolyticus, wyróżniąjąc na tej podstawie 11 profili genowych (kompozycji genów). Analizowano częstość występowania poszczególnych profili u poszczególnych gatunków gronkowców i enterokoków.

EN

Aminoglycoside modifying enzymes (AMEs) are major factors which confer aminoglycoside resistance on bacteria. Composition of six genes encoding AMEs (including lately described aph 2"- genes) was investigated by PCR for 16 clinical isolates of Enterococcus faecalis , 16 clinical isolates of coagulase-positive (S. aureus) and 13 clinical isolates of coagulase negative staphylococci (S. haemolyticus, S. epidermidis) collected in Gdańsk region (Northern Poland) in the years 1998-2001. Diversity of AME encoding gene profiles (composition) was used to analyze spread of AME encoding gene among and within studied group of cocci. According to presence of particular genes we distinguish eleven different AME encoding gene profiles: seven profiles were unique for particular species while the most com- mon was shared among S. aureus, coagulase negative staphylococci and enterococci. Regarding profile frequence statistical analysis (Fstat, AMOVA, cluster analysys UPGMA) shows: the difference between S. aureus and enterococci and coagulase-negative staphylococci, lack of difference between enterococci and coagulase-negative staphylococci, higher variability within than between studied species and presence of multispecies cluster. On the basisis of the reports about ability of staphylococci to synthesis enterococcal pheromones, this finding lets assume that spread of amminoglyciside resistance gene among gram (+) cocci is limited only by the ability of stains to synthesis or induction of synthesis conjugation protein.

Słowa kluczowe

PL

Enterococcus faecalis; antybiotyki aminoglikozydowe; gronkowce; acetylotransferaza; opornosc na antybiotyki; enzymy; nukleotydylotransferaza; fosfotransferaza; geny

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2003
• Tom: 55
• Numer: 4
• Opis fizyczny: s.303-308,rys.,tab.,bibliogr.

Twórcy

autor

Jarzembowski T.

• Akademia Medyczna, Gdansk

Bibliografia

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