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1996 | 43 | 3 |

Tytuł artykułu

Endoproteinase activities in wheat leaves upon water deficit

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
In wheat leaves acclimated and non-acclimated to water deficit the azocaseinolytic activities of endoproteinases were increased about 7-fold under drought conditions. Under such conditions both the pH dependence profile and the endoproteinase pattern were also changed. The predominant contribution of serine proteinase (about 50% of total endoproteinase activity) remains unaltered in the drought stressed leaves. Cysteine proteinase was induced to the same extent in the drought-stressed leaves irrespective of the acclimation pretreatment, while the contribution of aspartic proteinase was reduced upon water deficit but in the acclimated stressed leaves was as high as in the non-stressed leaves. These changes in the pattern of endoproteinases seem to imply that the water

Wydawca

-

Rocznik

Tom

43

Numer

3

Opis fizyczny

p.515-519,fig.

Twórcy

autor
  • Plant Breeding and Acclimation Institute, Radzikow, 00-950 Warsaw, Poland

Bibliografia

  • 1. Hanson, A.D. & Hitz, W.D. (1982) Metabolic responses of mesophytes to plant water defi­cits. Annu. Rev. Plant Physiol. 33,168-203.
  • 2. Ramagopal, S. (1987) Messenger RNA changes during drought stress in maize leaves. /. Plant Physiol. 129,311-317.
  • 3. King, S.W., Vierling, R.A. & Nguyen, H.T. (1992) Changes in mRNA species during drought stress in winter wheat. Crop Sci. 32,822-825.
  • 4. Close, T.J., Kortt, A. A. & Chandler, P.M. (1989) A cDNA-based comparison of dehydration- -induced proteins (dehydrins) in barley and com. Plant Mol. Biol. 13,95-108.
  • 5. Galani, G. A. & Close, T.J. (1992) Sequences of the cotton group 2L.EA/RAB/dehydrin proteins encoded bv lea 3 cDNAs. Plant Physiol. 98, 1523-1525.'
  • 6. Bray, E.A. (1993) Molecular responses to water deficit. Plant Physiol. 103,1035-1040.
  • 7. Vierstra, R.D. (1993) Protein degradation in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 44,385-410.
  • 8. Zagdariska, B. (1995) Respiratory energy de­mand for protein turnover and ion transport in wheat leaves upon water deficit. Physiol. Plant. 95,428-436.
  • 9. Stocker, O. (1929) Das Wasserdefizit von Gefasspflanzen in verschiedenen Klimazonen. Planta 7,382-387.
  • 10. Bradford, M.M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye-binding. Anal. Biochem. 72, 248-254.
  • 11. Dungey, N.O. & Davies, D.D. (1982) Protein turnover in isolated barley leaf segments and the effects of stress. J. Exp. Bot. 33,12-20.
  • 12. James, F., Brouquisse, R., Pradet, A. & Raymond, P. (1993) Changes in proteolytic activities in glucose-starved maize root tips. Regulation by sugars. Plant Physiol. Biochem. 31,845-856.
  • 13. Schaffer, M.A. & Fischer, R.L. (1990) Transcri­ptional activation by heat and cold of a thiol protease gene in tomato. Plant Physiol. 93,1486- -1491.
  • 14. Miller, B.L & Huffaker, R.C. (1981) Partial puri­fication and characterization of endo-protein- ases from senescing barley leaves. Plant Physiol. 68,930-936.
  • 15. Zagdariska, B. & Wtfniewski, K. (1996)Changes in the thiol /disulfide redox potential in wheat leaves upon water deficit. ). Plant Physiol, (in press)
  • 16. Lin, W. & Wittenbach, V.A. (1981) Subcellular localization of proteases in wheat and corn mesophyll protoplasts. Plant Physiol. 969-972.
  • 17. Wittenbach, V.A., Lin, W. & Hebert, R.R. (1982) Vacuolar localization of proteases and degra­dation of chloroplasts in mesophyll protoplasts from senescing primary wheat leaves. Plant Physiol. 69,98-102.
  • 18. Wildner, G.F. (1982) Ribulose-l,5-bisphosphate carboxylase-oxygenase: Aspects and prospects. Physiol. Plant. 52,385-389.
  • 19. Veierskov, B. & Ferguson, I.B. (1991) Ubiquitin conjugating activity in leaves and isolated chlo­roplasts from Avena sativa L. during senescence. Plant Physiol. 138,608-613.
  • 20. Peoples, M.B., Frith,G.J.T. & Dalling, M.J. (1979) Proteolytic enzymes in green wheat leaves. IV. Degradation of ribulose 1,5-bisphosphale carboxylase by acid proteinases isolated on DEAE-cellulose. Plant Cell Physiol. 20, 253-258.
  • 21. Bhalla, PL. & Dalling, M.J. (1986) Endopepti- dase and carboxypeptidase enzymes of vacuo­les prepared from mesophyll protoplasts of the primary leaf of wheat seedlings. J. Plant Physiol. 122, 289-302.
  • 22. Kervinen, J., Kontturi, M. & Mikola, J. (1990) Changes in the proteinase composition of barley leaves during senescence in field conditions. Cereal Res. Comm. 18,191-197.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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