Deletion-insertion polymorphism of the prion protein gene [PRNP] in Polish Holstein-Friesian cattle

• Autorzy: Czarnik U , Zabolewicz T. , Strychalski J. , Grzybowski G. , Bogusz M. , Walawski K.
• Języki publikacji: EN

Abstrakty

EN

The aim of the present study was to identify the deletion/insertion polymorphism of the bovine prion protein gene (PRNP) within the promoter sequence (23 bp), intron 1 (12 bp) and 3' untranslated region (14 bp). DNA was isolated from blood of 234 randomly tested Polish Holstein-Friesian cows and from semen of 47 sires used for artificial insemination (AI) in 2004. No statistically significant differences were found in the frequency of genotypes and alleles between cows and breeding bulls in the 3 analysed polymorphic sites within the PRNP gene. Only 3 haplotypes were identified in sires and 4 haplotypes in cows.

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2007
• Tom: 48
• Numer: 1
• Opis fizyczny: p.69-71,ref.

Twórcy

autor

Czarnik U

• University of Warmia and Mazury in Olsztyn, M.Oczapowskiego 5, 10-957 Olsztyn, Poland

autor

Zabolewicz T.

autor

Strychalski J.

autor

Grzybowski G.

autor

Bogusz M.

autor

Walawski K.

Bibliografia

• Don RH, Cox PT, Wainwright BT, Beker K, Mattick JS, 1991. Touchdown PCR to circumvent spurious priming during gene amplification. Nucl Acids Res 19: 4008.
• Excoffier L, Laval G, Schneider S, 2005. Arlequín ver. 3.0: An integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online 1: 47-50.
• Goldmann W, Hunter N, Martin T, Dawson H, Hope J, 1991. Different forms of the bovine PrP gene have five or six copies of a short G-C element within the protein-coding region. Gen Virol 72: 201-204.
• Hills D, Comincini S, Schlaepfer J, Dolf G, Ferretti L, Williams JL, 2001. Complete genomic sequence of the bovine prion gene (PRNP) and polymorphism in its promoter region. Anim Genet 32: 231-232.
• Horiuchi M, Ishiguro N, Nagasawa H, Toyoda Y, Shingawa H, 1998. Genomic structure of the bovine PrP gene and complete nucleoside sequence of bovine PrP cDNA. Anim Genet 29: 37-40.
• Leone P, Castiglioni B, Sechi T, Cassini P, Stella A, 2002. Prion gene octa-repeat variability in the Italian cattle breeds. Proc. 7th World Congress on Genetic Applied in Livestock Production. August 19-21, 2002, Montpellier, France: 13-40.
• Nakamitsu S, Miyazawa T, Horiuchi M, Onoe S, Ohoba Y, Kitagawa H, Ishiguro N, 2005. Sequence variation of bovine prion protein gene in Japanese cattle (Holstein and Japanese Black). J Vet Med Sci 68: 27-33.
• Prusiner SB, 1998. Prions. Proc Natl Acad Sci USA. 95: 13363-13383.
• Sander P, Hamann H, Drögemüller C, Kashkevich K, Schiebel K, Tosso Leeb T, 2005. Bovine prion protein gene (PRNP) promoter polymorphisms modulate PRNP expression and may be responsible for differences in bovine spongiform encephalopathy susceptibility. J Biol Chem 280: 37408-37414.
• Sander P, Hamann H, Pfeifer I, Wemheuer W, Breng B, Groschup MH, et al. 2004. Analysis of sequence variability of the bovine prion protein gene (PRNP) in German cattle breeds. Neurogenetics 5: 19-25.
• Seabury CH, Womack EJ, Piedrahita J, Derr NJ, 2004. Comparative PRNP genotyping of U.S. cattle for potential association with BSE. Mammalian Genome 15: 828-833.
• Walawski K, Czarnik U, 2003. Prion protein octapeptide-repeat polymorphism in Polish Black and White cattle. J Appl Genet 44: 191-195.