Cell cycle synchronization in the root meristem of Allium cepa L.

• Autorzy: Woloszyn M. , Golczyk H. , Joachimiak A.
• Języki publikacji: EN

Abstrakty

EN

A high level of cell cycle synchronization in Allium cepa was achieved using a 24 h hydroxyurea block (1.25 mM) followed by 20 h of recovery (without hydroxyurea) and a subsequent 4 h treatment with saturated α-bromonaphthalene solution. This procedure was very effective in producing a synchronously dividing cell population with a 76% mean mitotic index and a 30% frequency of metaphase stages. After release from hydroxyurea arrest, large fractions of cells synchronously entered successive phases: G1, S, and G2. The calculated duration of the cell cycle, 13 h, is in general accordance with previous reports. A detailed protocol for obtaining a high mitotic index and a large portion of cells in a particular cycle phase (G1, S, G2) was prepared.

Słowa kluczowe

EN

synchronization; Allium cepa; cell cycle; hydroxyurea; DNA measurement; alpha-bromonaphthalene; mitotic index; meristem; root

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2001
• Tom: 43
• Opis fizyczny: p.97-103,fig.,ref.

Twórcy

autor

Woloszyn M.

• Jagiellonian University, Grodzka 52, 31-044 Krakow, Poland

autor

Golczyk H.

autor

Joachimiak A.

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