Endotoxemia in newborn rats attenuates acute pancreatitis at adult age

• Autorzy: Jaworek J. , Konturek S.J. , Macko M. , Kot M. , Szklarczyk J. , Leja-Szpak A. , Nawrot-Porabka K. , Stachura J. , Tomaszewska R. , Siwicki A. , Pawlik W.W.
• Języki publikacji: EN

Abstrakty

EN

Bacterial endotoxin (lipopolysaccharide, LPS), at high concentration is responsible for sepsis, and neonatal mortality, however low concentration of LPS protected the pancreas against acute damage. The aim of this study was to investigate the effect of exposition of suckling rats to LPS on the course of acute pancreatitis at adult age. Suckling rat (30-40g) received intraperitoneal (i.p.) injection of saline (control) or LPS from Escherichia coli or Salmonella typhi (5, 10 or 15 mg/kg-day ) during 5 consecutive days. Two months later these rats have been subjected to i.p. caerulein infusion (25 µg/kg) to produce caerulein-induced pancreatitis (CIP). The following parameters were tested: pancreatic weight and morphology, plasma amylase and lipase activities, interleukin 1ß (IL-1 ß), interleukin 6 (IL-6), and interleukin 10 (IL-10) plasma concentrations. Pancreatic concentration of superoxide dysmutase (SOD) and lipid peroxidation products; malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) have been also measured. Caerulein infusion produced CIP in all animals tested, that was confirmed by histological examination. In the rats, which have been subjected in the neonatal period of life to LPS at doses 10 or 15 mg/kg-day x 5 days, all manifestations of CIP have been reduced. In these animals acute inflammatory infiltration of pancreatic tissue and pancreatic cell vacuolization have been significantly diminished. Also pancreatic weight, plasma lipase and a-amylase activities, as well as plasma concentrations of IL-1ß and IL-6 have been markedly decreased, whereas plasma anti-inflammatory IL-10 concentration was significantly increased in these animals as compared to the control rats, subjected in the infancy to saline injection instead of LPS. Caerulein-induced fall in pancreatic SOD concentration was reversed and accompanied by significant reduction of MDA + 4 HNE in the pancreatic tissue. The effects of LPS derived from E.coli or S.typhi were similar. Pretreatment of suckling rats with LPS at dose of 10 mg/kg-day x 5 days resulted in the most prominent attenuation of acute pancreatitis at adult age, whereas LPS at dose of 5 mg/kg-day x 5 days given to the neonatal rats failed to affect significantly acute pancreatitis induced in these animals 2 months later. We conclude that: 1/ Prolonged expositon of suckling rats to bacterial endotoxin attenuated acute pancreatitis induced in these animals at adult age. 2/ This effect could be related to the increased concentration of antioxidative enzyme SOD in the pancreatic tissue and to the modulation of cytokines production in these animals.

Słowa kluczowe

EN

acute pancreatitis; superoxide dismutase; interleukin; lipopolysaccharide; sepsis; suckling rat; concentration; endotoxemia; caerulein-induced pancreatitis; adult; rat; newborn

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2007
• Tom: 58
• Numer: 1
• Opis fizyczny: p.131-147,fig.,ref.

Twórcy

autor

Jaworek J.

• Jagiellonian University Collegium Medicum, Grzegorzecka Street 16, 31-531 Krakow, Poland

autor

Konturek S.J.

autor

Macko M.

autor

Kot M.

autor

Szklarczyk J.

autor

Leja-Szpak A.

autor

Nawrot-Porabka K.

autor

Stachura J.

autor

Tomaszewska R.

autor

Siwicki A.

autor

Pawlik W.W.