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2006 | 62 | 12 |

Tytuł artykułu

Dystrybucja wazoaktywnego peptydu jelitowego i neuropeptydu Y w neuronach zwojow ledzwiowo-krzyzowych pnia wspolczulnego swini

Warianty tytułu

EN
Distribution patterns of vasoactive intestinal peptide and NPY in the lumbar-sacral sympathetic chain ganglia of pigs

Języki publikacji

PL

Abstrakty

EN
The aim of the study was to determine the existence and co-existence patterns of VIP and NPY in neurons and nerve fibers of porcine lumbar-sacral sympathetic chain ganglia. The studied ganglia were fixed with 4% buffered paraformaldehyde (perfusion) and then labeled by means of double-immunofluorescence using a mixture of antibodies cultivated in different species. The highest number of NPY-positive cells was observed in the lumbar ganglia and diminished in the direction of the caudal, where only single neurons were observed. In contrast, a different pattern of distribution was observed for VIP-positive neurons, whose number was higher in the more caudally located ganglia. Two populations of VIP-positive neurons could be distinguished: single, showing strong immunofluorescence and often with visible processes, located in the central part of the ganglia and a second population, composed of clusters of 4-8 cells and often co-localizing NPY. VIP-positive nerve fibers surrounded both NPY+ neurons and neurons lacking either NPY and/or VIP. The presence of a small number of NPY-positive neurons exhibiting very weak immunofluorescence in more caudally located SChG could suggest a "switch" of neuromediators produced there. An increase in the percentage of non-noradrenergic sympathetic neurons in more caudally located SChG may thus be implicative for a specific innervation pattern of target tissues of these ganglia.

Wydawca

-

Rocznik

Tom

62

Numer

12

Opis fizyczny

s.1415-1419,fot.,bibliogr.

Twórcy

autor
  • Uniwersytet Warminsko-Mazurski, ul.Oczapowskiego 13, 10-719 Olsztyn
autor
autor
autor

Bibliografia

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  • 3.Chiba T., Masuko S.: Autonomic neurons and paraneurons (SIF cells) in the sympathetic ganglia regulating guinea pig proximal colon: immunohistochemical studies. Arch Histol Cytol. 1989, 52, 343-350.
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  • 10.Klimaschewski L., Obermuller N., Majewski M., Bachman S., Heym C.: Increased expression of nitric oxide synthase in a subpopulation of rat sympathetic neurons after axotomy correlation with vasoactive intestinal peptide. Cell Tissue Res. 1996, 285, 419-425.
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  • 12.Lindh B., Risling M., Remahl S., Terenuis L., Hoekfelt T.: Peptide-immunoreactive neurons and nerve fibres in lumbosacral sympathetic ganglia: selective elimination of a pathway-specific expression of immunoreactivities following sciatic nerve resection in kittens. Neuroscience 1993, 55, 545-562.
  • 13.Lindh B., Staines W., Hoekfelt T., Terenius L., Salvaterra P.: Immunohistochemical demonstration of choline acetyltransferase-immunoreactive preganglionic nerve fibers in guinea pig autonomic ganglia. Neurobiology 1986, 83, 5316-5320.
  • 14.Lundberg J., Hokfelt T., Schultzberg M., Uvnas-Wallenstein K., Kohler C., Said S.: Occurence of vasoactive intestinal poluypeptide (VIP)-like immunoreactivity in certain cholinergic neurons of the cat: evidence from combinated immunohistochemistry and acetylcholinesterase staining. Neuroscience 1979, 4, 1539-1559.
  • 15.£akomy M., Happola O., Kaleczyc J., Majewski M.: Immunohistochemical localization of neuropeptides in the porcine thoraco-lumbar paravertebral ganglia. Anat. Histol. Embryol. 1994, 23, 12-20.
  • 16.McLachlan E., Llewellyn-Smith I.: The immunohistochemical distribution of neuropeptide Y in lumbar pre- and paravertebral sympathetic ganglia of the guinea pig. J. Auton. Nerv. System 1986, 17, 313-324.
  • 17.Portbury A., McConalogue K., Furness J., Young H.: Distribution of pituitary adenyl cyclase activating peptide (PACAP) immunoreactivity in neurons of the guinea-pig digestive tract and their projections in the ileum and colon. Cell Tissue Res. 1995, 279, 385-392.
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  • 20.Schimosegawa T., Koizumi M., Toyota T., Goto Y., Kobayashi S., Yanaihara C., Yanaihara N.: Methionine-enkephalin-Arg6 -Gly7 -Leu8 -immunoreactive nerve fibers and cell bodies in lumbar paravertebral ganglia and the celiac-superior mesenteric ganglion complex of the rat: an immunohistochemical study. Neurosci. Lett. 1985, 57, 169-174.
  • 21.Trudrung P., Furness J., Pompolo S., Messenger J.: Locations and chemistries of sympathetic nerve cells that project to the gastrointestinal tract and spleen. Arch. Histol. Cytol. 1994, 57, 139-150.
  • 22.Wąsowicz K.: Changes in the expression of tyrosine hydroxylase (TH), dopamine beta-hydroxylase (DBH), galanin (GAL), vasoactive intestinal polypeptide (VIP) and substance P (SP) in the uterine cervix-projecting neurons located in the lumbar paravertebral ganglia of the pig. Folia Morphol. (Warsz). 2003, 62, 219-221.

Typ dokumentu

Bibliografia

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