Detection of damage-recognition proteins from human lymphocytes

• Autorzy: Lanuszewska J. , Cudak A. , Rzeszowska-Wolny J. , Widlak P.
• Języki publikacji: EN

Abstrakty

EN

Proteins recognizing and binding to damaged DNA (DDB-proteins) were analyzed in human lymphocytes obtained from healthy donors. Using an electrophoretic mobility shift assay several complexes between nuclear extract proteins and damaged DNA were detected: a complex specific for DNA damaged by N-acetoxy-N-acetylaminofluorene, another complex specific for UV-irradiated DNA, and two complexes specific for DNA damaged by cis-dichlorodiammine platinum. All the detected complexes differed in electrophoretic mobility and possibly contained different proteins. Complexes specific for free DNA ends were also detected in protein extracts from lymphocytes.

Słowa kluczowe

EN

man; DNA binding protein; damage recognition; lymphocyte; protein; detection

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2000
• Tom: 47
• Numer: 2
• Opis fizyczny: p.443-450,fig.

Twórcy

autor

Lanuszewska J.

• Center of Oncology, 44-100 Gliwice, Poland

autor

Cudak A.

autor

Rzeszowska-Wolny J.

autor

Widlak P.

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