Uptake and subcellular distribution of intraventricularly injected [1-3H]dolichol in rat brain

• Autorzy: Jankowski W. , Strosznajder J.
• Języki publikacji: EN

Abstrakty

EN

The uptake of C95[l-3H]dolichol in the form of liposomes into rat brain after the intracerebral and intraperitoneal injection was inves­tigated. Efficient, time-dependent uptake of dolichol into the brain was observed exclusively after the intraventricular injection. Within 24 h after the injection about 10% of the applied dolichol was found in the brain and 1.5% in liver. The distribution of dolichol in various parts of rat brain decreased in the order: cerebellum > midbrain > grey matter and brain stem > white matter. Sevtn days after the injection total radioactivity in the brain decreased and concomitantly a sig­nificant increase was observed in blood circulating and liver of the rat. The highest activity was found in grey matter and it remained a few times higher in comparison with that in whitematter. About 80% of the dolichol taken up by the brain membrane was recovered in the follow­ing subcellular fractions: crude nuclear fraction > microsomes > mitochondria > synaptosomes > myelin.

Słowa kluczowe

EN

brain; dolichol; rat; intraventricular injection; subcellular distribution

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1992
• Tom: 39
• Numer: 2
• Opis fizyczny: p.215-222,fig.

Twórcy

autor

Jankowski W.

• Institute of Biochemistry and Biophysics, Rakowiecka 36, 02-532 Warsaw, Poland

autor

Strosznajder J.

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