Integrin receptors play a role in the internalin B-dependent entry of Listeria monocytogenes into host cells

• Autorzy: Auriemma C , Viscardi M. , Tafuri S. , Pavone L.M. , Capuano F. , Rinaldi L. , Della Morte R. , Iovane G. , Staiano N.
• Języki publikacji: EN

Abstrakty

EN

Listeria monocytogenes enters non-phagocytic cells by binding its surface proteins inlA (internalin) and inlB to the host’s E-cadherin and Met, respectively. The two internalins play either separate or cooperative roles in the colonization of infected tissues. Here, we studied bacterial uptake into HeLa cells using an L. monocytogenes mutant strain (ΔinlA) carrying a deletion in the gene coding for inlA. The ΔinlA mutant strain showed the capability to invade HeLa cells. The monoclonal anti-β3- and anti-β1-integrin subunit antibodies prevented bacterial uptake into the cells, while the anti-β2- and anti-β4-integrin subunit antibodies failed to affect L. monocytogenes entry into HeLa cells. Three structurally distinct disintegrins (kistrin, echistatin and flavoridin) also inhibited bacterial uptake, showing different potencies correlated to their selective affinity for the β3- and β1-integrin subunits. In addition to inducing Met phosphorylation, infection of cells by the L. monocytogenes ΔinlA mutant strain promoted the tyrosine phosphorylation of the focal adhesion-associated proteins FAK and paxillin. Our findings provide the first evidence that β3- and β1-integrin receptors play a role in the inlB-dependent internalization of L. monocytogenes into host cells.

Słowa kluczowe

EN

integrin receptor; internalin B; Listeria monocytogenes; host cell; paxillin; human pathogen; focal adhesion kinase; bovine serum albumin; bovine fetal serum; food-borne pathogen; internalin A; sodium dodecyl sulphate

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2010
• Tom: 15
• Numer: 3
• Opis fizyczny: p.496-506,fig.,ref.

Twórcy

autor

Auriemma C

• Istituto Zooprofilattico Sperimentale del Mezzogiorno, via Della Salute 2, 80055, Portici, Italy

autor

Viscardi M.

autor

Tafuri S.

autor

Pavone L.M.

autor

Capuano F.

autor

Rinaldi L.

autor

Della Morte R.

autor

Iovane G.

autor

Staiano N.

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