Induction and characterization of streptomycin-resistant mutants in Capsicum praetermissum

• Autorzy: Venkataiah P , Christopher T , Subhash K
• Języki publikacji: EN

Abstrakty

EN

Streptomycin-resistant mutants were isolated from mutagenised cotyledon explants of Capsicum praetermissum Heiser & Smith. The expiants were mutagenised with N-ethyl-N-nitrosourea, which resulted in a high frequency of streptomycin-resistant mutants (18.0%) and a low frequency of chlorophyll-deficient (albino) mutants (8.0%). Complete streptomycin-resistant plantlets were obtained after rooting of the regenerated green shoots on rooting medium containing 1.0 mg L⁻¹ IAA and 500 mg L⁻¹ streptomycin sulphate. Leaf-segment assay of these plantlets revealed that they were resistant to streptomycin but sensitive to chloramphenicol, kanamycin, lincomycin, and spectinomycin. Reciprocal crosses between streptomycin-resistant and -sensitive plants showed a non-Mendelian transmission of resistance by female parents.

Słowa kluczowe

EN

plant biotechnology; mitochondrion; chloroplast; cotyledon culture; mutagenesis; mutant; maternal inheritance; Capsicum praetermissum; streptomycin resistance; streptomycin-resistant mutant; genetic information

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2005
• Tom: 46
• Numer: 1
• Opis fizyczny: p.19-24,ref.

Twórcy

autor

Venkataiah P

• Plant Biotechnology Lab, Department of Botany, Kakatiya University, Warangal, India

autor

Christopher T

• Plant Biotechnology Lab, Department of Botany, Kakatiya University, Warangal, India

autor

Subhash K

• Plant Biotechnology Lab, Department of Botany, Kakatiya University, Warangal, India

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