In vitro regeneration and somaclonal variation of Petunia hybrida

• Autorzy: Abu-Qaoud H , Abu-Rayya A. , Yaish S.

Warianty tytułu

PL

Zmiennosc somaklonalna podczas regeneracji in vitro pedow Petunia hybrida

• Języki publikacji: EN

Abstrakty

EN

The effect of different levels of naphthaleneacetic acid (NAA) and benzyladenine (BA) on shoot multiplication and regeneration of Petunia hybrida was studied. Regenerated shoots from leaf explants were grown ex vitro for analysis of somaclonal variation. Seeds of Petunia hybrida were germinated in vitro on MS basal medium. The seedlings were used as a source of explants for the multiplication and regeneration experiments. For the shoot multiplication experiment, shoot explants taken from germinated seedlings were cultured on MS basal media supplemented with different concentrations of BA (0.1, 0.4, 0.8 mgl-1) and NAA at concentration of 0.1 mg l-1. Highest number of axillary shoot was obtained on medium supplemented with 0.8 mg l-1 BA and 0.1 mgl-1NAA. For regeneration experiments, leaf sections taken from germinated seedlings were cultured onto MS media supplemented with three levels of BA (0.5, 1.0 and 2.0 mgl-1) and 0.5 mgl-1NAA. The highest shoot regeneration rate (45%) was observed in MS medium supplemented with 2 mg l-1 BA. For the evaluation of somaclonal variation, lateral bud explants were taken from pink colored petunia plants that were grown in the greenhouse. The buds were disinfected and cultured on MS basal media supplied with 30 mgl-1 gentamycin sulfate and 30 mg l-1 Benlate. After shoots grew, leaf sections were then taken from them shoots and cultured onto shoot regeneration medium (MS medium supplemented with 2 mg l-1 BA). The regenerated adventitious shoots were cultured on MS medium without growth regulator. These shoots were then rooted, acclimatized and transferred to the greenhouse for evaluation. Two forms of leaf shape (orbicular and elliptic) and three flower colors (violet, purple and light pink) appeared on the plantlets.

PL

Badano wpływ rożnych stężeń kwasu naftylooctowego (NAA) i benzyladeniny (BA) na regeneracj ę i namnażanie pędów Petunia hybryda. Występowanie zmienności somaklonalnej analizowano podczas wzrostu ex vitro pędów zregene­rowanych z eksplantatów liściowych. Nasiona Petunia hybrida kieIkowały in vitro na po żywce podstawowej MS. Siewki posłużyły jako źródło eksplantatów do do świad­czeń nad mikrorozmnażaniem i regeneracją pędów. W doświadczeniach nad mikro- rozmnażaniem eksplantaty pędowe pobrane z siewek byfy wyszczepiane na pożywkę MS z dodatkiem różnych stężeń BA (0,1, 0,4, 0,8 mgl-1) i NAA w stężeniu 0,1 mgl-1. Największą liczbę pędów kątowych uzyskano na pożywce z BA w stężeniu 0,8 mg l-1 i NAA 0,1 mg l-1. Do do świadczeń nad regeneracją pobierano liście z siewek, które następnie przecinano na pół i wszczepiano na pożywkę MS z dodatkiem BA w stężeniach 0,5, 1,0 i 2,0 mg l-1 oraz NAA w stężeniu 0,5 mg l-1. Największą efektywność regeneracji pędów (45%) stwierdzono na pożywce MS wzbogaconej w 2 mgl-1 BA. Do oceny zmienności somaklonalnej pobierano eksplantaty w postaci pąków bocznych z rosnących w szklarni roślin petunii o różowych kwiatach. Pąki były dezynfekowane i wszczepiane na pożywkę MS z dodatkiem 30 mg l-1 siarczanu gentamycyny i 30 mg l-1 Benlate. Gdy z pąków rozwinęły sięp ędy, pobierano z nich eksplantaty liściowe i wyszczepiano na pożywkę do regeneracji pędów (MS z dodatkiem 2 mgl-1 BA). Zregenerowane pędy przybyszowe były ukorzeniane, aklimatyzowane i wysadzone w szklarni dla oceny zmienności. W stosunku do formy wyjściowej, rośliny otrzymane z pędów przybyszowych miafy dwie nowe formy liści (kołowe i eliptyczne) oraz trzy nowe kolory kwiatów (fioletowe, purpurowe i jasnoróżowe).

Słowa kluczowe

EN

in vitro; regeneration; somaclonal variation; Petunia hybrida; seed; naphthaleneacetic acid; benzyladenine; shoot multiplication; leaf explant; tissue culture

• Wydawca: -
• Czasopismo: Journal of Fruit and Ornamental Plant Research
• Rocznik: 2010
• Tom: 18
• Numer: 1
• Opis fizyczny: p.71-81,fig.,ref.

Twórcy

autor

Abu-Qaoud H

• An Najah National University, P.O. Box 707. Nablus, Palestinian Authority

autor

Abu-Rayya A.

autor

Yaish S.

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