Linkage groups and the indirect chromosome location of cms-P-linked AFLPs

• Autorzy: Bednarek P T , Lewandowska R. , Kubicka H. , Masojc P.
• Języki publikacji: EN

Abstrakty

EN

Twenty-five AFLPs, previously linked to fertility restoration genes for the male-sterilizing PAMPA cytoplasm (cms-P) in a restricted rye population, were studied in an enlarged population of 120 plants. A strong association with the trait was verified for 19 of the markers. The recombination of these markers was tested and two linkage groups were identified: one consisting of six and the other of eight AFLPs. The remaining markers were segregated as independent loci. Using wheat-rye addition and substitution lines, the AFLPs were assigned to individual rye chromosomes. AFLP profiles of such lines were generated to identify the DNA fragments co-migrating with individual markers. This identified 1R and 3 R as the two chromosomes corresponding to the linkage groups of eight and six markers, respectively. Mapping in a DS2 x RXL10 population linked four additional AFLPs to chromosome arms 1RS, 3RL, 4RL and 6RL. RAPD and SSR markers mapped in various populations and known to be located on the appropriate chromosomes did not disrupt the C394-F2 population into sterile and fertile phenotypes. It was concluded that the identified markers would reduce by one half the number of primer pair combinations needed for molecular breeding programs or for the selection of parental forms for rye hybrid crosses.

Słowa kluczowe

EN

chromosome; rye; parental form; hybrid; breeding programme; molecular marker; DNA fragment; population; mapping; substitution line

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2002
• Tom: 07
• Numer: 2B
• Opis fizyczny: p.721-736,fig.

Twórcy

autor

Bednarek P T

• Polish Academy of Sciences, 02-973 Warsaw, Prawdziwka 2, Poland

autor

Lewandowska R.

autor

Kubicka H.

autor

Masojc P.

Bibliografia

• 1. Rafalski, A., Wiśniewska, I., Sikora, T. and Łapiński, B. Molecular probes for detection of wheat chromosomal fragments in rye. Plant Breed. 44 (2002) 17-38.
• 2. Anioł, A. and Gustafson, J. P. Chromosome location of genes controlling aluminium tolerance in wheat, rye and triticale. Can. J. Cytol. 26 (1984) 701-705.
• 3. Curtis, C. A. and Łukaszewski, A. J. Localization of genes in rye that restore male ferility to hexaploid wheat with timopheei cytoplasm. Plant Breed. 111 (1993) 106-112.
• 4. Plaschke, J., Börner, A., Xie, D. X., Koebner, R. M. D., Schlegel, R. and Gale, M. D. RFLP mapping of genes affecting plant height and growth habit in rye. Theor. Appl. Genet. 85 (1993) 1049-1054.
• 5. Miftahudin, G. J. Scoles and Gustafson, J. P. AFLP markers tightly linked to the aluminum-tolerance gene Alt3 in rye (Secale cereale L.). Theor. Appl. Genet. 104 (2002) 626-631.
• 6. Alonso-Blanco, C., Goicoechea, P. G., Roca, A., Alvarez, E. and Giraldez, R. Genetic mapping of cytological and izozyme markers on chromosomes 1R, 3R, 4R and 6R rye. Theor. Appl. Genet. 88 (1994) 208-241.
• 7. Philipp, U., Wehling, P. and Wricke, G. A linkage map of rye. Theor. Appl. Genet. 88 (1994) 243-248.
• 8. Senft, P. and Wricke, G. An extended genetic map of rye (Secale cereale L.). Plant Breed. 115 (1996) 508-510.
• 9. Devos, K. M. Rye map. http://wheat.pw.usda.gov/graingenes.html, (1996).
• 10. Gustafson, J. P. Linkage map of rye, UC90 x E-Line. http://wheat.pw.usda.gov.graingenes.html, (1996).
• 11. Schlegel, R., Melz, G. and Korzun, V. Genes, marker and linkage data of rye (Secale cereale L.): 5th updated inventory. Euphytica 101 (1998) 23-67.
• 12. Loarce, Y., Hueros, G. and Ferrer, E. A molecular linkage map of rye. Theor. Appl. Genet. 93 (1996) 1112-1118.
• 13. Korzun, V., Malyshev, S., Voylokov, A. V. and Börner, A. A genetic map of rye (Secale cereale L.) combining RFLP, isozyme, protein, microsatellite and gene loci. Theor. Appl. Genet. 102 (2001) 709-717.
• 14. Masojć, P., Myśków, B. and Milczarski, P. Extending a RFLP -based genetic map of rye using random amplified polymorphic DNA (RAPD) and isozyme markers. Theor. Appl. Genet. 102 (2001) 1273-1279.
• 15. Saal, B. and Wricke, G. Development of simple sequence repeat markers in rye (Secale cereale L.). Genome 42 (1999) 964-972.
• 16. Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, J. A. and Tingey, S. V. DNA polymorphisms amplified by arbitrary primer usful as genetic markers. Nucleic Acids Res. 18 (1990) 577-586.
• 17. Vos, P., Hogers, R., Bleeker, M., Lee, T. van, Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M. and Zauber, M. AFLP: A new technique for DNA fingerprinting. Nucleic Acids Res. 23 (1995) 4407-4414.
• 18. Glass, C. J. Chromosomale Lokalisation von Genen fuhr die Restauration der männlichen Fertilität mit RFLP-Markern bei der Winterroggenlinie L18. Dissertation, Univ. (1997) Stuttgart.
• 19. Dreyer, F. Chromosomale Lokalisation von Rostorergenen argentinischer und iranischer Herkunft in F2-Populationen von Winterroggen (Secale cereale L.). Dissertation, Univ. Stuttgart (1999).
• 20. Miedaner, T., Glass, C., Dreyer, F., Wilde, P., Wortmann, H. and Geiger, H. H. Mapping of genes for male-fertility restoration in 'Pampa' CMS winter rye (Secale cereale L.). Theor. Appl. Genet. 101 (2000) 1226-1233.
• 21. Glass, C., Miedaner, T. and Geiger, H. H. Localisation von Restorergenen der Winterroggenline L18 mit RFLP-Markern. Vortr. Pflanzuchtung 31 (1995) 52-55.
• 22. Reinborn, H. Kartirung von Restorergenen mit RFLP-Markern in einer F2-Population von Winterroggen. Dipl.arb. Univ. Hannover, Germany (1995).
• 23. Dreyer, F., Miedaner, T. and Geiger, H. H. Chromosomal localization of male-fertility restorer genes from Argentinian and Iranian rye. Vortr. Pflanzenzuchtung 35 (1996) 280-281.
• 24. Wricke, G., Wilde, P., Wehling, P., and Gieselmann, C. An isozyme marker for pollen fertility restoration to Pampa cms system of rye (Secale cerealae L.). Plant Breed. 111 (1993) 290-294.
• 25. Miedaner, T., Dreyer, F., Glass, C., Reinbold, H. and Geiger, H. H. Mapping of genes for pollen fertility restoration in rye (Secale cereale L.). Vortr. Pflanzenzuechtg 38 (1997) 303-314.
• 26. Melz, G. and Adolf, K. Genetic analysis of rye (Secale cereale L.). Genetics of male sterility of the G-type. Theor. Appl. Genet. 82 (1991) 761-764.
• 27. Adolf, K. and Winkel, A. A new source of spontaneous sterility in winter rye. Preliminary results. Proc. Eucaria Meet. Cereal Sect. Rye. Svalov, Sweeden, (1985) 293-307.
• 28. Börner, A., Korzun, V., Polley, A., Malyshev, S. and Melz, G. Genetics and molecular mapping of a male-fertility restoration locus (Rfg1) in rye (Secale cereale L.). Theor. Appl. Genet. 97 (1998) 99-102.
• 29. Bednarek, P. T., Kubicka, H., Zawada, M. and Brukwiński, W. Application of the AFLP markers for genetic variability testing within parental form of a rye hybrid based on the Pampa cytoplasm Biul. Inst. Hod. Aklim. Rośl. 211 (1999) 229-237.
• 30. Bednarek, P. T., Kubicka, H., Zawada, M. and Brukwiński, W. Bulk analysis of pseudo segregating F1 plants of rye hybrid based on cms Pampa by the AFLP technique. Biul. Inst. Hod. Aklim. Rośl. 216 (2000) 101-110.
• 31. Michelmore, R. W., Paran, I. and Kesseli, R. V. Identification of markers linked to desease resistance genes by bulk segregant analysis: a rapid method to detect markers in specific genomic region by using segregating populations. Proc. Natl. Acad. Sci. USA 88 (1991) 9828-9832.
• 32. Bednarek, P. T., Kubicka, H. and Zawada, M. Morphological, cytological and BSA-based testing on limited segregation population AFLPs. Cell. Mol. Biol. Let., this issue.
• 33. Voort, J. R. van, Zandvoort, P. van, Eck, H. J. van, Folkertsma, R. T., Hutten, R. C. B., Draaistra, J., Gommers, F. J., Jacobsen, E., Helder, J. and Bakker, J. Use of allele specificity of comigrating AFLP markers to align genetic maps from different potato genotypes. Mol. Gen. Genet. 255 (1997) 438-447.
• 34. Geiger, H. H. and Morgenstern, K. Angewandt-genetische Studien zur cytoplasmatischen Pollenstarilitat bei Winterroggen. Theor. Appl. Genet. 46 (1975) 269-276.
• 35. Yuan, Y. Umweltsensibilität der cytoplasmisch-genisch vererbten männlichen Sterilität (CMS) bei Roggen (Secale cereale L.). Dissertation, Univ. (1996) Stuttgart.
• 36. Ukai, Y. http://wheat.ab.a.u-tokyo.ac.jp/~ukai. (2000).
• 37. Lincoln, S. E., Daly, M. J. and Lander, E. S. Constructing genetic linkage maps with MAPMAKER/Exp v.3.0. A tutorial and reference manual. Third edition, (1993).
• 38. Voort, J. R. van, Wolters, P., Folkertsma, R., Hutten, R., Zandvoort, P. van, Vinke, H., Kanyuka, K., Bendahmane, A., Jacobsen, E., Janssen, R. and Bakker, J. Mapping of the cyst nematode resistance locus Gpa2 in potato using a strategy based on comigrating AFLP markers. Theor. Appl. Genet. 95 (1997) 874-880.
• 39. Waugh, R., Bonar; N., Baird, E., Thomas, B., Graner, A., Hayes, P. and Powell, W. Homology of AFLP products in three mapping populations of barley. Mol. Gen. Genet. 255 (1997) 311-321.
• 40. Miller, T. E. and Reader, S. M. A guide to homoeology of chromosomes within the Triticeae. Theor. Appl. Genet. 74 (1987) 214-217.
• 41. Korzun, V., Malyshev, S., Kartel, N., Westermann, T., Weber, W. E. and Börner, A. A genetic linkage map of rye (Secale cereale L.). Theor. Appl. Genet. 96 (1998) 203-208.
• 42. Meksem, K., Ruben, E., Hyten, D., Triwitayakorn, K. and Lightfoot, D. A. Conversion of AFLP bands into high-throughput DNA markers. Mol. Genet. Genomics 265 (2001) 207-214.