Cloning of the DNA fragments reflecting the open reading frame I and II of the I-18 C gene of Chironomus tentans. I. Preparation of the bacterial cells, transformation and isolation of the bluescript plasmid

• Autorzy: Borowicz B P
• Języki publikacji: EN

Abstrakty

EN

The technology applied for the cloning of the open reading frame (ORF) I and II reflecting DNA fragments of the I-18 C gene of Chironomus tentans in regard to the preparation of the competent E. coli cells of the XL-1 strain needed for the transformation with the ligation reaction products, is presented. Also the transformation of these bacterial cells with the bluescript plasmid and its isolation for these cloning experiments, are described.

PL

Opisano technologię, którą zastosowano do klonowania fragmentów DNA odzwierciedlających translacyjną otwartą ramę odczytu I i II genu I-18 C Chironomus tentans w zakresie: przygotowania kompetentnych komórek E. coli szczepu XL-1, poddawanych następnie transformacji produktami reakcji ligacji. Przedstawiono także zastosowaną technologię transformacji wymienionych komórek bakteryjnych przy użyciu plazmidu - blueskrypt oraz izolację tego plazmidu, w celu jego zastosowania w wymienionych eksperymentach klonowania.

Słowa kluczowe

EN

transformation; promoter system; gene expression; cloning; I-18 C gene; isolation; T7 RNA polymerase; plasmid; Chironomus tentans; bacterial cell; polymerase chain reaction; DNA fragment

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 1999
• Tom: 39
• Numer: 1
• Opis fizyczny: p.39-45

Twórcy

autor

Borowicz B P

• Institute of Plant Protection, Miczurina 20, 60-318 Poznan, Poland

Bibliografia

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