Embryo-callus regenerated hybrids and amphidiploids of barley cultivars x rye inbred lines

• Autorzy: Wojciechowska B , Pudelska H.
• Języki publikacji: EN

Abstrakty

EN

In the presented hybridization programme of barley cultivars and rye inbred lines including 48 cross combinations the seed set ranged from 3.13 to 92.98%, while embryos were formed in 0.74 to 36.36% in successful pollinations. Sixty five plants were generated by embryo callus culture and one - by embryo culture without callus formation. The hybrids had somatic chromosome numbers 2n=14 (60 plants) and 2n=28 (6 plants). Plants obtained via embryo callus culture showed good vegetative vigour and well-developed root system. Spike morphology of all plants resembled that of rye. Meiosis in 17 diploids showed 0.13-0.63 barley-barley and rye-rye bivalents with a chiasma frequency of 0.14-0.69 per cell. The heteromorphic bivalent-like configurations occurred in five plants in 0.01-0.02 per cell. The amphidiploids had 7.79-10.71 barley-barley and rye-rye bivalents with a chiasma frequency of 9.36-17.75 per cell. All plants, with 14 and 28 chromosomes, were completely sterile both in backcrosses and when selfed.

Słowa kluczowe

EN

rye; embryo culture; barley; intergeneric hybrid; meiotic pairing

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 1995
• Tom: 36
• Numer: 4
• Opis fizyczny: p.313-323,fig.

Twórcy

autor

Wojciechowska B

• Polish Akademy of Sciences, 60-479 Poznan, Strzeszynska 34, Poland

autor

Pudelska H.

Bibliografia

• FEDAK O. (1977). Barley monoploids and hybrids from barley × rye crosses. Interspecific Hybridization in Plant Breeding. Proc. 8th Congress of Eucarpia, Madrid: 269-273.
• FEDAK G. (1979). Cytogenetics of a barley × rye hybrid. Can. J. Genet. Cytol. 21: 543-548.
• FEDAK G. (1984). Cytogenetics of tissue culture regenerated hybrids of Triticum tauschii × Secale cereale. Can. J. Genet. Cytol. 26: 382-386.
• FEDAK G. (1986). Hordecale (Hordeum vulgare L. × Secale cereale L.). In: Biotechnology in Agriculture and Forestry, Vol. 2: Crops I, (Y.P.S. Bajaj, ed.) Springer-Verlag: 544-555.
• FEDAK O. (1992). Intergeneric hybrids with Hordeum. In: Barley: Genetics, Biochemistry, Molecular Biology and Biotechnology (P.R. Shewry, ed.). Biotechnology in Agriculture Series, No 5, C.A.B. International: 45-70.
• FINCH R.A., SMITH J.B., BENNETT M.D. (1981). Hordeum and Secale mitotic genomes lie apart in a hybrid. J. Cell Sci. 52: 391-403.
• FUJIGAKI J., TOZU T. (1993). Reproductive barriers between Hordeum and Secale species. Wheat Inf. Serv. 76: 59-60.
• GAMBORG O.L., MILLER R.A., OJIMA K. (1968). Nutrient requirements of suspension cultures of soyabean root cells. Exp. Cell Res. 50: 151-158.
• KRUSE A. (1967). Intergeneric hybrids between Hordeum vulgare L. ssp. distichum (v. Pallas, 2n=14) and Secale cereale L. (v. Petkus, 2n=14). Royal Vet. and Agric. Coll. Yearbook, Copenhagen: 82-92.
• KRUSE A. (1976). Reciprocal hybrids between the genera Hordeum, Secale and Triticum. Hereditas 84: 224.
• LEVAN A. (1942). Studies on the meiotic mechanism of haploid rye. Hereditas 28: 177-211.
• LINDE-LAURSEN I. (1991). Barley × rye hybrids eliminating accessory chromosomes of rye. Hereditas 115: 127-132.
• MURASHIGE T., SKOOG F. (1962). A revised medium for rapid growth and bioassays with tabacco tissue cultures. Physiol. Plant. 15: 473-497.
• NORDENSKIÖLD H. (1939). Studies of a haploid rye plant. Hereditas 25: 204-210.
• SASAKI M. (1987). Triticale cytogenetics and breeding. Japan. J. Breed. 37 (Suppl. 1): 2-5.
• SHARMA H.C., GILL B.S. (1983). Current status of wide hybridization in wheat. Euphytica 32: 17-31.
• THOMAS H.M., PICKERING R.A. (1979). Barley × Rye crosses. The morphology and cytology of the hybrids and the amphidiploids. Z. Pflanzenzüchtg. 82: 193-200.
• WOJCIECHOWSKA B. (1984). Crosses of barley with rye, Hordeum jubatum × 4x Secale cereale and BC progenies of H. jubatum × 2x S. cereale. Cereal Res. Commun. 12: 67-73.
• WOJCIECHOWSKA B. (1985). Hybrids between barley and alloplasmic rye. Genet. Pol. 26: 317-326.
• WOJCIECHOWSKA B. (1987). Barley haploids and hybrids regenerated from callus tissue of Hordeum vulgare L. and Secale cereale L. embryos. Genet. Pol. 28: 217-223.
• WOJCIECHOWSKA B., PONITKA A., ŚLUSARKIEWICZ-JARZINA A. (1984). In vitro propagation of the barley-rye and barley-wheat hybrids. Int. Symp. Plant Tissue and Cell Culture Application to Crop Improvement. Olomouc, September 24-29, 1984, Proceedings: 459-460.
• WOJCIECHOWSKA B., PUDELSKA H. (1992). Intergeneric hybrids of Hordeum vulgare L. × Secale cereale L. regenerated from embryo callus culture. Genet. Pol. 33: 87-96.