Defective brca2 influences topoisomerase I activity in mammalian cells

• Autorzy: Rahden-Staron I. , Szumilo M. , Grosicka E. , Kraakman-van der Zwet M. , Zdzienicka M.Z.
• Języki publikacji: EN

Abstrakty

EN

The Chinese hamster cell mutant V-C8 is defective in the Brca2 gene (Kraakman-van der Zwet et al., 2002, Cell Biol.-, 22: 669). Here we report that V-C8 cells were 10-fold more sensitive to camptothecin, an inhibitor of topoisomerase I, than the parental V79 cells. The level of the relaxation activity of topoisomerase I in nuclear extracts was also lower (4-fold) in V-C8 than V79 cells, in spite of the fact that the level of the topoisomerase I protein was the same in these cells. The survival of V-C8 cells in the presence of camptothecin, the sensitivity of V-C8 topoisomerase I to camptothecin, and the level of the relaxation activity in V-C8 nuclear extract were almost completely restored by transfection of V-C8 cells with the murine Brca2 gene or by the transfer of human chromosome 13 providing the BRCA2 gene. These results indicate that the ob­served changes in the topoisomerase I activity in V-C8 are due to the defective func­tion of the Brca2 gene.

Słowa kluczowe

EN

cell mutant; Chinese hamster; Brca2 gene; topoisomerase I; mammalian cell; camptothecin

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2003
• Tom: 50
• Numer: 1
• Opis fizyczny: p.139-144,fig.

Twórcy

autor

Rahden-Staron I.

• Medical University of Warsaw, S.Banacha 1, 02-097 Warsaw, Poland

autor

Szumilo M.

autor

Grosicka E.

autor

Kraakman-van der Zwet M.

autor

Zdzienicka M.Z.

Bibliografia

• Bradford MM. (1976) AnalBiochem.; 72: 248-54.
• Bernstein C, Bernstein H, Payne CM, Garewald H. (2002) MutatRes.; 511: 145-78. Boothman DA, Trask DK, Pardee AB. (1989) Cancer Res.; 49: 605-12.
• Boothman DA, Wang M, Schea R, Burrows HL, Strickfaden S, Owens JK. (1992) Int J Radiat Oncol Biol Phys.; 24: 939-48.
• Champoux JJ. (2001) Annu Rev Biochem. ; 70: 369-413.
• Chaung W, Mi L-J, Boorstein R. (1997) Nucleic Acids Res.; 25: 992-4.
• D'Arpa P, Liu LF. (1995) Exp Cell Res.; 217: 125-31.
• Davies SM, Robson CN, Davies SL, Hickson JD. (1988) J Biol Chem.; 263: 17724-9. Davies SM, Davies SL, Hall AG, Hickson JD. (1990) Mutat Res.; 235: 111-8. Ferguson LR, Baguley BC. (1994) Environ Mol Mutagen. ; 24: 245-61. Ferro AM, Higgins NP, Olivera BM. (1983) J Biol Chem.; 258: 6000-3. Frankenberg-Schwager M, Frankenberg D. (1990) Int J Radiat Biol.; 58: 569-75. Gupta M, Fujimori A, Pommier Y. (1995) Biochim Biophys Acta.; 1262: 1-14. Johnstone A, McNerney R. (1985) Biosci Rep.; 5: 907-12. Karran P. (2000) Curr Opin Genet Dev.; 10: 144-50.
• Kim JM, Kim SH, Kolozsvary A, Khil MS. (1992) Int J Radiat Oncol Biol Phys.; 22: 515-8.
• Kraakman-van der Zwet M, Overkamp WJ, van Lange RE, Essers J, van Duijn-Goedhart A, Wiggers I, Swaminathan S, van Buul PP, Errami A, Tan RT, Jaspers NG, Sharan SK, Kanaar R, Zdzienicka MZ. (2002) Mol Cell Biol.; 22: 669-79.
• Liu LF. (1983) Meth Enzymol.; 100: 133-7.
• Overkamp WJ, Rooimans MA, Neuteboom I, Telleman P, Arwert F, Zdzienicka MZ. (1993) Somat Cell Mol Genet. ; 19: 431-7.
• Pommier Y, Kerrigan D, Hartman KD, Glazer RI. (1990) J Biol Chem.; 265: 9418-22.
• Pommier Y, Leteurtre F, Fesen MR, Fujimori A, Bertrand R, Solary E, Kolhagen G, Kohen W. (1994) Cancer Invest.; 12: 530-42.
• Rahman N, Stratton MR. (1998) Annu Rev Genet.; 32: 95-121.
• Sambrook J, Fritsch E, Maniatis T. (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbour Laboratories, Cold Spring Harbour, NY.
• Slichenmayer WJ, Rowinsky EK, Donehower RC, Kaufman SH. (1993) J Natl Cancer Inst.; 85: 271- 91.
• Thielmann HW, Popanda O, Gersbach H, Gilberg F. (1993) Carcinogenesis.; 14: 2341-51.
• Trzcinska AM, Girstun A, Piekielko A, Kowalska-Loth B, Staron K. (2002) Mol Biol Rep.; 29: 347-52.
• Verhaegh GW, Jongmans W, Morolli B, Jaspers NG, van der Schans GP, Lohman PH, Zdzienicka MZ. (1995) Mutat Res.; 337: 119-29.
• Wang JC. (2002) Nat Rev Mol Cell Biol.; 3: 430-40. Watt PM, Hickson ID. (1994) Biochem J; 303: 681-95. Weaver DT. (1996) Crit RevEukaryot Gene Expr.; 6: 345-75. Xia F, Powell SN. (2002) Semin Radiat Oncol.; 12: 296-304.
• Xia F, Taghian DG, DeFrank JS, Zeng Z-C, Willers H, Itiakis G, Powell SN. (2001) Proc Natl Acad Sci US A.; 98: 8644-9.
• Zdzienicka MZ. (1995) Mutat Res.; 336: 203-13.