Loop formation by the transgene WAP:6xHishGH in transgenic rabbit fibroblasts, revealed by fluorescence in situ hybridization to nuclear halos

• Autorzy: Michalak E , Lipinski D , Slomski R
• Języki publikacji: EN

Abstrakty

EN

Using fluorescence in situ hybridization (FISH) to somatic nuclear halos from transgenic rabbits WAP:6xHishGH, we present evidence for stability of transgenesis at the chromatin level. FISH performed on fibroblasts from a homozygous individual showed 2 independent loops from both chromosomes of pair 7. On a heterozygous individual, FISH detected a single loop. According to the concept of chromatin loops and their influence on gene expression, this shows that the human growth hormone transgene, which was actively expressed in mammary gland under the influence of the tissue-specific promoter, was inactive in examined skin fibroblasts.

Słowa kluczowe

EN

chromosome; animal genetics; gene expression; chromatin level; skin fibroblast; growth hormone; fluorescence in situ hybridization; tissue-specific promoter; mammary gland; fibroblast; rabbit; transgenic rabbit

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2006
• Tom: 47
• Numer: 3
• Opis fizyczny: p.247-249,fig.,ref.

Twórcy

autor

Michalak E

• Department of Biochemistry and Biotechnology, Agricultural University, Wolynska 35, 60-637 Poznan, Poland

autor

Lipinski D

• Institute of Human Genetics, Polish Academy of Sciences, Poznań, Poland

autor

Slomski R

• Department of Biochemistry and Biotechnology, Agricultural University, Wolynska 35, 60-637 Poznan, Poland

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