The evaluation of methods for detection of Bacillus anthracis spores in artificially contaminated soil samples

• Autorzy: Bielawska-Drozd A , Niemcewicz M. , Bartoszcze M.
• Języki publikacji: EN

Abstrakty

EN

The nested PCR has been used to evaluate the usefulness and efficiency of different Bacillus anthracis spore isolation methods in contaminated soil samples. The best results were obtained using two methods described by Beyer et al. [1] and Cheun et al. [9]. Outer and inner pairs of primers were designed from the protective antigen gene of plasmid pXO1 as well as from genes B and C of the capsule region of the plasmid pXO2. The influence of soil types on obtained results was also studied. The type of soil samples did not affect the nested PCR results. Furthermore, the sensitivity of nested PCR and PCR – ELISA was also examined.

Słowa kluczowe

EN

Bacillus anthracis; detection method; spore; nested polymerase chain reaction; ELISA test; soil sample; soil contamination; soil type; contaminated soil

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2008
• Tom: 17
• Numer: 1
• Opis fizyczny: p.5-10,fig.,ref.

Twórcy

autor

Bielawska-Drozd A

• Institute of Hygiene and Epidemiology, 2 Lubelska St., 24-100 Pulawy, Poland

autor

Niemcewicz M.

autor

Bartoszcze M.

Bibliografia

• 1. BEYER W., GLÖCKNER P., OTTO J., BÖHM R. A nested PCR method for the detection of Bacillus anthracis in environmental samples collected from former tannery sites. Microbiol. Res. 150, 179, 1995.
• 2. BEYER W., GLÖCKNER P., OTTO J., BÖHM R. A nested PCR and DNA – amplification – fingerprinting method for detection and identification of Bacillus anthracis in soil samples from former tanneries. Salisbury Med. Bull. Special Suppl. 87, 47, 1996.
• 3. BEYER W., POCIVALSEK S., BÖHM R. Polymerase chain reaction – ELISA to detect Bacillus anthracis from soil samples – limitations of present published primers. J. Appl. Microbiol. 87 (2), 229, 1999.
• 4. DZIUBEK Z. Infectious and parasitic diseases. PZWiL Medical Publisher Warsaw pp. 102–103, 1996, [In Polish].
• 5. KOBUCH E., DAVIS J., FLEISCHER K., ISAACSON M., TURNBULL P. C. B. A clinical and epidemiological study of 621 patients with anthrax in western Zimbabwe. Salisbury Med. Bull. Special Suppl. 68, 34, 1990.
• 6. MATRAS J., BARTOSZCZE M. Bacillus anthracis. Post. Mikrob. 41 (1), 3, 2002. [In Polish]
• 7. TITBALL R. W., TURNBULL P. C. B., HUTSON R. A. The monitoring and detection of Bacillus anthracis in the environment. J. Appl. Bact. Symp. Suppl. 70, 9S, 1991.
• 8. ASAKURA H., MAKINO S. I., SHIRAHATA T., TSUKAMOTO T., KURAZONO H., IKEDA T., TAKESHI K. Detection and long – term existence of shiga toxin (stx) producing Escherichia coli in sheep. Microbiol. Immuol. 42 (10), 683, 1998.
• 9. CHEUN H. I., MAKINO S. I., WATARAI M., ERDENEBAATAR J., KAWAMOTO K., UCHIDA I. Rapid and effective detection of anthrax spores in soil by PCR. J. Appl. Microbiol. 95 (4), 728, 2003.
• 10. LUSZNIEWICZ A., SŁABY T. Statistics with STATISTICATM PL software. Theory and practice C. H. Beck Publishing House; Warszawa, pp. 222 – 227, 2001. [In Polish].
• 11. TRUSZCZYŃSKI M. Veterinary bacteriology. PWRiL Publisher, Warsaw, pp. 442 – 454, 1984. [In Polish]
• 12. JOHNS M., HARRINGTON L., TITBALL R. W., LESLIE D. L. Improved methods for the detection of Bacillus anthracis spores by the polymerase chain reaction. Lett. Appl. Microbiol. 18, 236, 1994.
• 13. RYU C., LEE K., YOO C., SEONG W., OH H. Sensitive and rapid quantitative detection of anthrax spores isolated from soil samples by real – time PCR. Microbiol. Immunol. 47 (10), 693, 2003.
• 14. YEATES C., GILLINGS M. R., DAVISON A. D., ALTAVILLA N., VEAL D. A. Methods for microbial DNA extraction from soil for PCR amplification. Biological Procedures Online 1 (1), 40, 1998.
• 15. DRAGON D. C., RENNIE R. P. Evaluation of spore extraction and purification methods for selective recovery of viable Bacillus anthracis spores. Lett. Appl. Microbiol. 33, 100, 2001.
• 16. CHEUN H. I., MAKINO S. I., WATARAI M., SHIRAHATA T., UCHIDA I., TAKESHI K. A simple and sensitive detection system for Bacillus anthracis in meat and tissue. J. Appl. Microbiol. 91 (3), 421, 2001.
• 17. TEBBE C. C., VAHJEN W. Interference of humic acids and DNA extracted directly from soil in detection and transformation of recombinant DNA from bacteria and yeast. Appl. Environ. Microbiol. 59 (8), 2657, 1993.
• 18. GRZYBOWSKI J., REISS J. The textbook of practical medical and sanitary bacteriology. Bellona Publishing House, Warsaw, pp. 172 – 75, 2001. [In Polish]
• 19. MAZUREK J. Anthrax – The actual threat. Słuz. Zdr. – Pol Med J, 86/87, 1, 2001. [In Polish].
• 20. TURNBULL P. C. B., BÖHM R., COSIVI O., DOGANAY M., HUGH – JONES M. E., DE VOS V. Guidelines for the Surveillance and Control of Anthrax in Humans and Animals, WHO/EMC/ZDI/98/6. 1998.