Engrafting fetal liver cells into multiple tissues of healthy adult mice without the use of immunosuppressants

• Autorzy: Darinskas A , Gasparaviciute R. , Malisauskas M. , Wilhelm K. , Kozhevnikov J.A. , Liutkevicius E. , Pilinkiene A. , Morozova-Roche L.A.
• Języki publikacji: EN

Abstrakty

EN

We have shown the fetal liver cell engraftments into multiple tissues of adult healthy mice, achieved without suppressing the animals’ immune systems. Fetal cells from the livers of male C57Bl/6J Black lineage mice at day 13 to 15 of gestation were injected intravenously into female adult CC57W/MY White mice. The grafting was evaluated by Y-chromosome-specific PCR, cytometric analysis of fluorescently stained donor cells, and histological analysis. All the methods consistently showed the presence of multiple engraftments randomly distributed through the various organs of the recipients. After 60 days, the grafts still constituted 0.1 to 2.75% of the tissues. The grafted cells did not change their appearance in any of the organs except the brain, where they became enlarged. Inflammatory reactions were not detected in any of the histological preparations. The frequency of engraftments was higher in the liver, indicating that similarity between the donor and recipient cells facilitates engraftment. The high inherent plasticity of fetal liver cells underlies their ability to integrate into healthy recipient organs, which can be governed by environmental conditions and connections with neighboring cells rather than by the initial cellular developmental programs. The fact that fetal liver cells can be grafted into multiple tissues of healthy animals indicates that they can be used to replace the natural loss of cells in adult organisms.

Słowa kluczowe

EN

fetal cell; cell; mice; damaged tissue; immune system; healthy mice; animal; regeneration; transplantation; adult mice; mouse; immunosuppressant; immune suppression; liver; tissue

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2007
• Tom: 12
• Numer: 3
• Opis fizyczny: p.422-434,fig.,ref.

Twórcy

autor

Darinskas A

• Vilnius University, Vilnius 08409, Lithuania

autor

Gasparaviciute R.

autor

Malisauskas M.

autor

Wilhelm K.

autor

Kozhevnikov J.A.

autor

Liutkevicius E.

autor

Pilinkiene A.

autor

Morozova-Roche L.A.

Bibliografia

• 1. Bhattacharya, N. Fetal cell/tissue therapy in adult disease: a new horizon in regenerative medicine. Clin. Exp. Obstet. Gynecol. 31 (2004) 167-173.
• 2. Snyder, B.J. and Olanow, C.W. Stem cell treatment for Parkinson's disease: an update for 2005. Curr. Opin. Neuro. 18 (2005) 376-385.
• 3. Melone, M.A., Jori, F.P. and Peluso, G. Huntington's disease: new frontiers for molecular and cell therapy. Curr. Drug Targets 6 (2005) 43-56.
• 4. Haider, H.Kh. and Ashraf, M. Bone marrow stem cell transplantation for cardiac repair. J. Physiol. Heart Circ. Physiol. 288 (2005) 2557-2567.
• 5. O’Donoghue, K., and Fisk, N.M. Fetal stem cells. Best Pract. Res. Clin. Obstet. Gynaecol. 18 (2004) 853-875.
• 6. Sembeil, R., Sanhadji, K., Vivier, G., Chargui, J. and Touraine, J.L. Prolonged survival of mouse skin allografts after transplantation of fetal liver cells transduced with hIL-10 gene. Transpl. Immunol. 13 (2004) 1-8.
• 7. de Vries-van der Zwan, A., van der Pol, M.A., de Waal, L.P. and Boog, C.J. An alternative conditioning regimen for induction of specific skin graft tolerance across full major histocompatibility complex barriers. Transpl. Immunol. 6 (1998) 147-151.
• 8. Wakabayashi, A., Eishi, Y. and Nakamura, K.J. Development of the immune system in severe combined immunodeficiency mice reconstituted with transferred fetal liver cells. Med. Dent. Sci. 44 (1997a) 21-29.
• 9. Taylor, P.A., McElmurry, R.T., Lees, C.J., Harrison, D.E. and Blazar, B.R. Allogenic fetal liver cells have a distinct competitive engraftment advantage over adult bone marrow cells when infused into fetal as compared with adult severe combined immunodeficient recipients. Blood 99 (2002) 1870-1872.
• 10. Sanhadji, K., Touraine, J.L., Aitouche, A., Vicari, A., Chargui, J. and Goillot, E. Fetal liver cell transplantation in various murine models. Bone Marrow Transplant. 9 (1992) 77-82.
• 11. Savitz, S.I., Dinsmore, J., Wu, J., Henderson, G.V., Stieg, P. and Caplan, L.R. Neurotransplantation of fetal porcine cells in patients with basal ganglia infarcts: a preliminary safety and feasibility study. Cerebrovasc. Dis. 21 (2005) 101-107.
• 12. Wakabayashi, A., Eishi, Y. and Nakamura, K. Regulation of experimental autoimmune orchitis by the presence or absence of testicular antigens during immunological development in SCID mice reconstituted with fetal liver cells. Immunology 92 (1997b) 84-90.
• 13. Chargui, J., Moya, M.J., Sanhadji, K., Blanc-Brunat, N. and Touraine, J.L. Anti-NK antibodies injected into recipient mice enhance engraftment and chimerism after allogeneic transplantation of fetal liver stem cells. Thymus 24 (1997) 233-246.
• 14. Yuh, D.D., Gandy, K.L., Hoyt, G., Reitz, B.A. and Robbins, R.C. Tolerance to cardiac allografts induced in utero with fetal liver cells. Circulation 94 (1996) 11304-11307.
• 15. Schoeberlein, A., Holzgreve, W., Dudler, L., Hahn, S. and Surbek, D.V. Tissue-specific engraftment after in utero transplantation of allogeneic mesenchymal stem cells into sheep fetuses. Am. J. Obstet. Gynecol. 192 (2005) 1044-1052.
• 16. Shields, L.E., Lindton, B., Andrews, R.G. and Westgren, M. Fetal hematopoietic stem cell transplantation: a challenge for the twenty-first century. J. Hematotherapy Stem Cell Res. 11 (2002) 617-631.
• 17. Chang, K.T., Sefc, L., Psenak, O., Vokurka, M. and Necas, E. Early fetal liver readily repopulates B lymphopoiesis in adult bone marrow. Stem Cells 23 (2005) 230-239.
• 18. Nisbet-Brown, E. and Diener, E. T lymphocytes from irradiation chimeras repopulated with 13-day fetal liver cells recognize antigens only in association with self-MHC products. J. Mol. Cell Immunol. 2 (1986) 235-242.
• 19. Rabinowich, H., Umiel, T. and Globerson, A. T-cell progenitors in the mouse fetal liver. Transplantation 35 (1983) 40-48.
• 20. Christensen, J.L., Wright, D.E., Wagers, A.J. and Weissman, I.L. Circulation and chemotaxis of fetal hematopoietic stem cells. PLoS Biology 2 (2004) 0368-0377.
• 21. Dainiak, N. and Ricks, R.C. The evolving role of haematopoietic cell transplantation in radiation injury: potentials and limitations. BJR Suppl. 27 (2005) 169-174.
• 22. Feng, R.Q., Du, L.Y. and Guo, Z.Q. In vitro cultivation and differentiation of fetal liver stem cells from mice. Cell Res. 15 (2005) 401-405.
• 23. Morrison, S.J., Uchida, N. and Weissman, I.L. The biology of hematopoietic stem cells. Annu. Rev. Cell Dev. Biol. 11 (1995) 35-71.
• 24. Drukker, M., Katz, G., Urbach, A. Characterization of the expression of MHC proteins in human embryonic stem cells. Proc. Natl. Acad. Sci. 99 (2002) 9864-9869.
• 25. Tatebe, M., Nakamura, R., Kagami, H., Okada, K. and Ueda, M. Differentiation of transplanted mesenchymal stem cells in a large osteochondral defect in rabbit. Cytotherapy 7 (2005) 520-530.
• 26. Chu, K., Park, K.I. and Lee, S.T. Combined treatment of vascular endothelial growth factor and human neural stem cells in experimental focal cerebral ischemia. Neurosci. Res. 53 (2005) 384-390.
• 27. Seglen, P.O. Preparation of isolated rat liver cells. Methods Cell Biol. 13 (1976) 29-83.
• 28. Zheng, Y.W., Ohkohchi, N. and Taniguchi, H. Quantitative evaluation of long-term liver repopulation and the reconstitution of bile ductules after hepatocellular transplantation. World J. Gastroenterol. 39 (2005) 6176-6181.
• 29. Wang, L.J., Chen, M.Y. and George, D. Engraftment assessment in human and mouse liver tissue after sex-mismatched liver cell transplantation by real-time quantitative PCR for Y-chromosome sequences. Liver Transpl. 9 (2002) 822-828.
• 30. Lapidot, T. and Petit, I. Current understanding of stem cell mobilization: the roles of chemokines, proteolytic enzymes, adhesion molecules, cytokines, and stromal cells. Exp. Hematol. 30 (2002) 973-981.