Electrochemical gradients across Malpighian tubules of the house cricket, Acheta domesticus, and the mode of action of kinin and CRF-related diuretics

• Autorzy: Coast G.M.
• Języki publikacji: EN

Abstrakty

EN

Double-barrelled ion-selective microelectrodes were used to measure Na+, K+ and Cl- activities in the principal cells of cricket Malpighian tubules, allowing electrochemical gradients across apical and basal membranes to be calculated. At the basal membrane, K+ and Cl- gradients support passive exit into the bathing medium. A thermodynamic analysis shows both ions can be taken up by Na+/K+/2Cl- cotransport driven by a favourable Na+ gradient. At the apical membrane, cations are actively transported into the lumen whereas Cl- moves passively. Evidence is presented to show that diuretic kinins open an apical membrane Cl- conductance whereas Achdo-DH acts via cAMP to stimulate the basal membrane NKCC.

Słowa kluczowe

EN

diuretic hormone; ion transport; insect; Acheta domesticus; electrochemical gradient; house cricket; fluid secretion; Malpighian tubule

• Wydawca: -
• Czasopismo: Pestycydy
• Rocznik: 2009
• Numer: 1-4
• Opis fizyczny: p.27-32,fig.,ref.

Twórcy

autor

Coast G.M.

• School of Biological and Chemical Sciences, Birkbeck, London WC1E 7HX, U.K.

Bibliografia

• [1] Ramsay J.A., J. Exp. Biol., 1954, 31, 104-113.
• [2] Maddrell S.H.P., O’Donnell M.J., ibid., 1992, 172, 417-429.
• [3] Coast G.M., Orchard I., Phillips J.E., Schooley D.A., Adv. Insect Physiol., 2002, 29, 279-409.
• [4] Coast G.M., Nachman R.J., Schooley D.A., J. Exp. Biol., 2007, 210, 3979-3989.
• [5] Ianowski J.P., Christensen R.J., O’Donnell M.J., ibid., 2002, 205, 1645-1655.
• [6] Coast G.M., Kay I., ibid., 1994, 187, 225-243.