In vitro regeneration of the Croatian endemic species Iris adriatica Trinajstic ex Mitic

• Autorzy: Keresa S , Mihovilovic A. , Curkovic-Perica M. , Mitic B. , Baric M. , Vrsek M. , Marchetti S.
• Języki publikacji: EN

Abstrakty

EN

Plant regeneration via somatic embryogenesis and organogenesis was achieved in leaf base and ovary culture of the Croatian endemic Iris adriatica Trinajstić ex Mitić. Callus induction from leaf base explants occurred in the dark on three media with MS mineral solution containing 4.52 μM dichlorophenoxyacetic acid (2,4-D), 4.83 μM naphthaleneacetic acid (NAA), 0.46 μM kinetin (Kin), 5% sucrose and 200 mg L-1 casein hydrolysate. The media differed only in vitamin and/or proline content. Calli from ovary culture were achieved on MS medium containing 45.25 μM 2,4-D. The mean percentage of callus induction from leaf base explants was 18.9%, with no significant differences between media, and 27.3% from ovary sections. All embryogenic calli were formed on MS media containing 0.45 μM 2,4-D, 4.44 μM benzyladenine (BA) and 0.49 μM indole-3-butyric acid (IBA) under low light intensity (25 μE m-2s-1). Transfer of embryogenic calli to hormone-free medium enabled the development of mature somatic embryos on the surface of 6.0% of induced calli produced from leaf base explants and 4.0% of those from ovary sections. Genotype had the main effect on plant regeneration efficiency in Iris adriatica

Słowa kluczowe

EN

in vitro culture; plant regeneration; Croatia; endemic species; Iris adriatica; Iridaceae; new species; leaf; ovary; somatic embryogenesis

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2009
• Tom: 51
• Numer: 2
• Opis fizyczny: p.7-12,fig.,ref.

Twórcy

autor

Keresa S

• University of Zagreb, Marulicev trg 20/II, 1000 Zagreb, Croatia

autor

Mihovilovic A.

autor

Curkovic-Perica M.

autor

Mitic B.

autor

Baric M.

autor

Vrsek M.

autor

Marchetti S.

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