Wplyw deksametazonu na wybrane steroidy plciowe i biochemiczne wskazniki w nasieniu ogierow po podaniu LPS

• Autorzy: Danek J

Warianty tytułu

EN

Effect of dexamethasone on some sex steroids and biochemical parameters in stallion semen following LPS administration

• Języki publikacji: PL

Abstrakty

EN

The aim of the study was to determine the effect of dexamethasone injections on changes in seminal plasma testosterone and oestradiol-17b concentration and biochemical characteristics in stallions following endotoxin administration. Three clinically healthy stallions were infused intravenously with endotoxin (LPS) from Escherichia coli 055:B5 at a dose of 0.3 mg/kg b.w.; while four stallions were treated with dexamethasone (0.069 mg/kg b.w., IM) 10 min before the administration of LPS. Endotoxin administration elicited (24h-9 weeks after LPS administration) a significant (P < 0.05) decrease in seminal plasma testosterone (T) and an increase in oestradiol-17β E₂β concentration. Administering endotoxin also influenced the seminal plasma biochemical components including total protein (TPR), albumin (ALB), total cholesterol (CHOL) concentration, and aspartate aminotransferase (AST) activity. A significant (P < 0.05) increase in TPR, ALB, and AST was noted. Dexamethason administration (DEX) had a different effect on endotoxin and induced testosterone and oestradiol-17β changes in the seminal plasma and biochemical quality of the stallion’s semen. DEX treatment had no influence on T, E₂β. The positive influence of DEX was noticed in TPR and AST. The negative influence of administering LPS+DEX in the group of stallions which only received LPS was noted particularly in ALB and CHOL.

Słowa kluczowe

PL

ogiery; testosteron; hormony plciowe; 17-beta-estradiol; jakosc; LPS; stezenie hormonow; endotoksyny; wskazniki biochemiczne; deksametazon; leki przeciwzapalne; nasienie; konie

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2007
• Tom: 63
• Numer: 03
• Opis fizyczny: s.348-352,rys.,bibliogr.

Twórcy

autor

Danek J

• Panstwowy Instytut Weterynaryjny - Panstwowy Instytut Badawczy w Pulawach, Oddzial w Bydgoszczy, al.Powstancow Wlkp. 10, 85-090 Bydgoszcz

Bibliografia

• 1. Barth A. D., Bowman P. A.: The sequential appearance of sperm abnormalities after scrotal insulation or dexamethasone treatment in bulls. Can. Vet. J. 1994, 35, 93-102.
• 2. Braun, J., Muto, Y., Sato, K., Schallenberger, E.: Der Einfluß von Jahreszeit und sexueller Beanspruchung auf die Konzentration von Testosteron und Östradiol- 17β im Seminalplasma beim Hengst. Tierärztl. Prax. 1996, 24, 577-580.
• 3. Danek J.: Biochemical changes in seminal plasma after endotoxin injection in stallions. Bull. Vet. Inst. Pulawy 2000, 44, 193-199.
• 4. Danek J.: Effect of dexamethasone treatment on the biochemical characteristicts of the stallion semen. Bull. Vet. Inst. Pulawy 2001, 45, 275-280.
• 5. Danek J.: Effect of Escherichia coli endotoxin on the levels of testosterone and estradiol-17β in blood serum and seminal plasma and on the semen characteristics in the stallion. Bull. Vet. Inst. Pulawy 2003, 47, 191-209.
• 6. Danek J.: Effects of administration of Escherichia coli lipopolysaccharides and flunixin meglumine on the semen quality in the stallion. Bull. Vet. Inst. Pulawy 2004, 48, 421-426.
• 7. Danek J.: Wpływ deksametazonu i fluniksyny na stężenie testosteronu i 17β-estradiolu w surowicy krwi oraz na morfologiê plemników ogiera. Medycyna Wet. 2004, 60, 1329-1332.
• 8. Danek J.: Wpływ deksametazonu na stężenie testosteronu, estronu i 17β-estradiolu oraz na biochemiczne wskaźniki w nasieniu ogierów. Medycyna Wet. 2005, 61, 335-337.
• 9. Danek J.: Wpływ lipopolisacharydu i fluniksyny na steroidy płciowe i biochemiczne wskaźniki nasienia ogierów. Medycyna Wet. (w druku).
• 10. Danek J.: Effects of flunixin meglumine on selected clinicopathologic variables, and serum testosterone concentration in stallions after endotoxin administration. J. Vet. Med. A. (w druku)
• 11. Ewert K. M., Fessler J. F., Templeton C. B., Bottoms G. D., Latshaw H. S., Johnson B. S.: Endotoxin-induced hematologic and blood chemical changes in ponies: Effects of flunixin meglumine, dexamethasone, and prednisolone. Am. J. Vet. Res. 1985, 46, 24-30.
• 12. Frauenfelder H. C., Fessler J. F., Moore A. B., Bottoms G. D., Boon G. D.: Effects of dexamethasone on endotoxin shock in the anesthetized pony: hematologic, blood gas, and coagulation changes. Am. J. Vet. Res. 1982, 43, 405-411.
• 13. Gow R. M., O’Bryan M. K., Canny B. J., Ooi G. T., Hedger M. P.: Differential effects of dexamethasone treatment on lipopolysaccharide-induced testicular inflammation and reproductive hormone inhibition in adult rats. J. Endocrinol. 2001, 168, 193-201.
• 14. Hess R. A., Thurston R. J.: Protein, cholesterol, acid phosphatase and aspartate aminotransaminase in the seminal plasma of turkeys (Meleagris gallopavo) producing normal white or abnormal yellow semen. Biol. Reprod. 1984, 31, 239-243.
• 15. Huacuja L., Delgado N. M., Calzada L., Wens A., Reyes R., Pedron N., Rosado A.: Exchange of lipids between spermatozoa and seminal plasma in normal and pathological human semen. Arch. Androl. 1981, 7, 343-349.
• 16. Juhasz J., Nagy P., Kulesar M., Huszenicza, Gy.: A review: Factors influencing semen quality and endocrinological sex function in stallions-with emphasis on glucocorticoids. Folia Vet. 2001, 45, 3-8.
• 17. Klaus A. M., Hapke H. J.: Natürliche und synthetische Glukokortikoide beim Sportpferd: eine Literaturübersicht. Dt. tierärztl. Wschr. 1996, 103, 489-528.
• 18. Luboshitzky R., Kaplan-Zverling M., Shen-Orr Z., Nave R., Herr P.: Seminal plasma androgen/oestrogen balance in infertile men. Int. J. Androl. 2002, 25, 345-351.
• 19. Lane P. J., Lees P.: Action of dexamethasone in an equine model of acute non-immune inflammation. Res. Vet. Sci. 1990, 48, 87-95.
• 20. Monn K. H., Bunge R. G.: Observation on the biochemistry of human semen. 4. Cholesterol. Fertil. Steril. 1970, 21, 80-83.
• 21. Morris D. D., Moore J. N., Cowe N., Fischer J. K.: Dexamethasone reduces endotoxin-induced tumor nectrosis factor activity production in vitro by equine peritoneal macrophages. Cornell. Vet. 1991, 81, 267-275.
• 22. O'Bryan M. K., Schlatt S., Phillips D. J., Krester D. M., Hedger M. P.: Bacterial lipopolysaccharide-induced inflammation compromises testicular function at multiple levels in vivo. Endocrinol. 2000, 141, 238-246.
• 23. Papadopoulos V., Drosdowsky M. A., Carreau S.: In vitro effects of prolactin and dexamethasone on rat Leydig cell aromatase activity. Andrologia 1986, 18, 79-83.
• 24. Saez J. M., Morera A. M., Haour F., Evain D.: Effects of in vivo administration of dexamethasone, corticotropin and human chrionic gonadotropin on steroidogenesis and protein and DNA synthesis of testicular interstitial cells in prepubertal rats. Endocrinol. 1977, 101, 1256-1263.
• 25. Sofikitis N., Miyagawa I.: Secretory dysfunction of the male accessory genital glands due to prostatic infections and fertility; a selected review of the literature. Jpn. J. Fertil. Steril. 1991, 36, 690-699.
• 26. Strzeżek J., Bieguszewski H., Szuperski T.: Wskaźniki biochemiczne w zastosowaniu do oceny jakości nasienia zwierząt gospodarskich. II. Badania nad przenikaniem albumin -J123 z krwi do plazmy nasiennej. Medycyna Wet. 1970, 26, 480-484.
• 27. Strzeżek J.: Wybrane układy enzymatyczne nasienia zwierząt gospodarskich w aspekcie doskonalenia jego konserwacji oraz płodności samca. Zesz. Probl. Post. Nauk. Rol. 1987, 340, 9-40.
• 28. Strzeżek J., Fraser L., Wysocki P., Kordan W., Hołody D., Dobrzyńska B.: Accessory sex glands of boar: inhibition of their secretory activity by antiandrogen cyproterone acetate. Fertitität 1998, 13, 244-250.
• 29. Templeton C. B., Bottoms G. D., Fessler J. F., Ewert K. M., Roesel O. F., Johnson M. A., Latshaw H. S.: Endotoxin-induced hemodynamic and prostaglandin changes in ponies: effects of flunixin meglumine, dexamethasone and prednisolone. Circ. Shock 1987, 23, 231-240.
• 30. Valsa J., Skandhan K. P., Umarvanshi V.: Cholesterol in normal and pathological seminal plasma. Panminerva Med. 1992, 34, 160-162.
• 31. Wallgren M.: Clinical, endocrinological and spermatological studies after endotoxin injection in the boar. J. Vet. Med. 1989, 36, 664-675.
• 32. Wallgren M., Kinndahl H., Larsson K.: Clinical, endocrinological and spermatological studies after endotoxin in the ram. J. Vet. Med. 1989, 36, 90-103.
• 33. Wallgren M., Kindahl H., Rodriguez-Martinez H.: Alterations in testicular function after endotoxin injection in the boar. Inter. J. Androl. 1993, 16, 235-243.
• 34. Wolfe D. F., Bradley J. T., Riddell M. G.: Characterization of seminal plasma proteins and sperm proteins in ejaculates from normospermic bulls and bulls with thermally-induced testicular degeneration. Theriogenology 1993, 40, 1083-1091.
• 35. Zalata A., Hafez T., Verdonck L., Vermeulen L., Comhaire F.: Androgens in seminal plasma: markers of the surface epithelium of male reproductive tract. Inter. J. Androl. 1995, 18, 271-277.