Biological control of apples blue mold by isolates of Saccharomyces cerevisiae

• Autorzy: Gholamnejad J , Etebarian H.R. , Roustaee A. , Sahebani N.A.

Warianty tytułu

PL

Biologiczne zwalczanie niebieskiej plesni jablek izolatami Saccharomyces cerevisiae

• Języki publikacji: EN

Abstrakty

EN

Yeasts (52, 51, 69, and 04) were received from Biotechnology Center of Karaj and Penicillium expansum Isolates P11 and P12 isolated from Golden Delicious. Isolates were evaluated as a potential biological control agents of apple blue mold caused by P. expansum. Dual culture, cell free metabolites and volatile test were used in vitro assay. All tested of yeast isolates inhibited growth of P. expansum. The inhibition varied among isolates of yeasts and ranged from 19.81% to 40.73%, in dual culture, from 43.16% to 66.44% in volatile metabolite and from 22.16% to 50.23% in cell free metabolite test. Apple fruit wounds were inoculated with 40 μl of yeast cell suspension (107cell/ml) followed 48 h later by P. expansum (105 conidia/ ml). The apples were then incubated at 25°C. Four isolates of Saccharomyces cerevisiae reduced, the decay area from 13.46 to 24.92 cm2 compared to 32.18 cm2 in control after incubation for 14 days at 25°C. At 5°C, the lesion size ranged from 13.58 to 24.68 cm2 for the antagonist treatments compared to 22 cm2 for the control treatments after 32 days. The isolate 69 of S. cervisiae was the most effective isolate at both tempetures in this assay and could be one of important new biological control agents for apple blue mold.

PL

Drożdże (52, 51, 69 i 04) otrzymano z centrum Biotechnologii Karaj, a Penicillium expansum izolowano z Golden Delicious. Izolaty oceniano jako potencjalne czynniki biologicznego zwalczania niebieskiej pleśni jabłek wywoływanej przez P. expansum. W testach in vitro uwzględniono podwójne kultury, bezkomórkowe metabolity i związki lotne. Wszystkie testowane izolaty drożdży inhibitowały wzrost P. expansum. Inhibicja różniła się w ramach testowanych izolatów i wahała się w granicach 19,81 do 40,73% w podwójnych kulturach, wynosiła od 43,16 do 66,44% w teście ze związkami lotnymi i od 22,16 do 50,23% w teście nie uwzględniającym metabolitów. Rany na jabłkach inokulowano 40 μl zawiesiny komórek drożdzy (107 komórek/ml), a po 48 godzinach P. expansum (105 komórek/ml). Następnie jabłko inkubowano w 25°C. Cztery izolaty P. expansum redukowały powierzchnię zgnilizny w zakresie od 13,46 do 24,92 cm2 w porównaniu do 32,18 cm2 w kontroli, po inkubacji przez 14 dni w 25°C. Po 32 dniach inkubacji w 5°C wielkość ran wynosiła od 13,58 do 24,68 cm2 dla kombinacji z traktowaniem antagonistą, w porównaniu do 22 cm2 w kontroli. Izolat 69 S. cerevicae był najbardziej efektywny w obydwóch temperaturach tego testu i mógłby być ważnym, nowym czynnikiem biologicznego zwalczania niebieskiej pleśni jabłek.

Słowa kluczowe

PL

biological control; blue mould; apple; Saccharomyces cerevisiae; isolate; Penicillium expansum; fruit; loss; fungal disease

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2009
• Tom: 49
• Numer: 3
• Opis fizyczny: p.270-275,fig.,ref.

Twórcy

autor

Gholamnejad J

• University of Tehran, Tehran, Iran

autor

Etebarian H.R.

autor

Roustaee A.

autor

Sahebani N.A.

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