The effect of substituted amides of pyrazine-2-carboxylic acids on flavonolignan production in Silybum marianum culture in vitro

• Autorzy: Tumova L , Gallova K. , Rimakova J. , Dolezal M. , Tuma J.
• Języki publikacji: EN

Abstrakty

EN

In vitro plant tissue and cell cultures were used to study herbicide effects on growth, selected metabolic activities and other phenomena. The effect of abiotic elicitors, two newly synthesized substituted amides of pyrazine-2-carboxylic acids (synthesized at the Department of Pharmaceutical Chemistry and Drug Control, School of Pharmacy in Hradec Kralove), on the flavonolignan accumulation in callus and suspension culture of Silybum marianum (L.) Gaertn. was investigated. The compounds markedly influenced production of flavonolignans in an in vitro culture. Particularly after the elicitation with a solution of compound 3-methylamide 5-tert-butylpyrazine- -2-carboxylic acid at a concentration of 3.71x10-7 moll-1 and within 72 h of elicitation, an increase in flavonolignan production by 893 % in suspension culture versus control took place. The flavonolignan accumulation in callus culture after the elicitation with a solution of 5-brom-2-hydroxyphenylamide of 5-tert-butyl-6-chloropyrazine-2-carboxylic acid was also increased by about 1039 % (24 hour elicitation and concentration of 2.59x10-4 moll-1 ).

Słowa kluczowe

EN

metabolic activity; flavonolignan production; pyrazine-2-carboxylic acid; amide; plant tissue; growth; in vitro; cell culture; Silybum marianum; abiotic elicitor; herbicide effect

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2005
• Tom: 27
• Numer: 3B
• Opis fizyczny: p.357-362,fig.,ref.

Twórcy

autor

Tumova L

• Charles University in Prague, Heyrovskeho 1203, 500 05 Hradec Kralove, Czech Republic

autor

Gallova K.

autor

Rimakova J.

autor

Dolezal M.

autor

Tuma J.

Bibliografia

• Blein J. P. 1982. Action of some herbicides on growth, respiration, plasmalemma integrity and proton extrusion of Acer pseudoplatanus cells. II. Amides, diphenyh ethers, nitriles, phenols triazines, and uracils. Pesticid. Biochem. Physiol. 17: 156-161.
• Davidonis G. H., Hamilton H. and Mumma R. O. 1978. Metabolism of 2,4-dichlorophenoxyacetic acid in soybean root callus and differentiated soybean root cultures as a function of concentration and tissue age. Plant Physiol. 62: 80-83.
• Dolezal M., Miletin M., Kunes J., Kralova K. 2002. Substituted amides of pyrazine-2-carboxylic acids. Synthesis and biological activity. Molecules 3: 363-373.
• Dwight Camber N., McDonald S. K. 1989. Tissue and cell cultures as model system in herbicide research. Rev. Weed Sci. 4: 169-190.
• Flora K., Hahn M., Rosen H., Benner K. 1998. Milk Thistle (Silybum marianum) for the therapy of liver disease. Am. J. Gastroenterol. 2: 139-143.
• Huffman J. B., Camber N. D. 1978. Growth inhibition in tobacco (Nicotiana tabacum) caltus by 2,6-dinit troaniline herbicides and protection by D-a-tocopherol acetate. Weed Sci. 26: 527-530.
• Linsmaier E. , Skoog F. 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant 18: 100-127.
• Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 437-497.
• Ricci D., Maggiali C. A., Ronchini F., Tirillini B., Fraternale, D. 1991. Auxin activity of diazinecarb t oxylic acids. Phytochemistry 30: 2821
• Schmitz R. V., Skoog F., Hecht S. M., Leonard, N. J. 1972. Comparison of zeatin indoleacetate with zeatin and indoleacetic acid in tobacco biosassay. Plant Physiol. 50: 114-116.
• Team of Authors 1993. Cardui mariae fructus. Czech Pharmaceutical Codex. X-EGEM, Prague.
• Tumova L., Ostrozlik P. 2002. Ononis arvensis in vi- tro-abioticka elicitace. Czech. Slov. Pharm. 4: 173-176.
• Wichtl M. 1997. Herbal Drugs and Phytopharmaceuticals. Medpharm Scientific Publishers, Stuttgart: 121-123.