Assessment of viral and non-viral gene transfer into adult rat brains using HSV-1, calcium phosphate and PEI-based methods

• Autorzy: Corso T D , Torres G. , Goulah C. , Roy I. , Gambino A.S. , Nayda J. , Buckley T. , Stachowiak E.K. , Bergey E.J. , Pudavar H. , Dutta P. , Bloom D.C. , Bowers W.J. , Stachowiak M.K.
• Języki publikacji: EN

Abstrakty

EN

CNS gene transfer could provide new approaches to the modelling of neurodegenerative diseases and devising potential therapies. One such disorder is Parkinson’s disease (PD), in which dysfunction of several different metabolic processes has been implicated. Here we review the literature on gene transfer systems based on herpes simplex virus type 1 (HSV-1) and non-viral polyethyleneimine (PEI) and calcium phosphate nanoparticle methods. We also assess the usefulness of various CNS gene delivery methods and present some of our own data to exemplify such usefulness. Our data result from vectors stereotaxically introduced to the substantia nigra (SN) of adult rats and evaluated 1 week and/or 1 month post injection using histochemical methods to assess recombinant β-galactosidase enzyme activity. Gene transfer using PEI or calcium phosphate-mediated transfections was observed for both methods and PEI was comparable to that of HSV-1 amplicon. Our data show that the amplicon delivery was markedly increased when packaged with a helper virus and was similar to the expression profile achieved with a full-size replication-defective HSV-1 recombinant (8117/43). We also examine whether PEI or HSV-1 amplicon-mediated gene transfer could facilitate assessment of the biological effects induced by a dominant negative FGF receptor-1 mutant to model the reduced FGF signalling thought to occur in Parkinson’s disease.

Słowa kluczowe

EN

assessment; viral gene; non-viral gene; adult; rat; brain; herpes simplex virus type 1; calcium phosphate; PEI-based method; Parkinson's disease; fibroblast growth factor; gene transfection; tyrosine kinase; adenovirus; beta-galactosidase; polyethylenimine

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2005
• Tom: 64
• Numer: 3
• Opis fizyczny: p.130-144,fig.,ref.

Twórcy

autor

Corso T D

• University at Buffalo, Suny, USA

autor

Torres G.

autor

Goulah C.

autor

Roy I.

autor

Gambino A.S.

autor

Nayda J.

autor

Buckley T.

autor

Stachowiak E.K.

autor

Bergey E.J.

autor

Pudavar H.

autor

Dutta P.

autor

Bloom D.C.

autor

Bowers W.J.

autor

Stachowiak M.K.

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