Industrial feed mixtures with an addition of methionine hydroxy analog

• Autorzy: Matyka S. , Rubaj J. , Korol W. , Bielecka G.
• Języki publikacji: EN

Abstrakty

EN

HMB (2-hydroxy-4-(methythio)butanoic acid), which is the precursor of methionine in the organism, is widely used in the production of industrial feeds as an addition supplementing the deficiency of methionine proper. HMB does not have any amino group, which is the reason why it cannot be marked within the analysis of the aminoacidic composition using the reaction with ninhydrine reagent. In the method of marking HMB using HPLC technique the conditions of chromatographic division were modified. The applied system of chromatographic columns and movable and eluating phases enabled effective separation of HMB from the elements of differentiated matrixes of industrial feed mixtures. The analytical proceedings are characterized by good validation parameters, i.e. high selectivity, high repeatability and a high degree of recovery of contents occurring in the production practice of industrial feeds ranging from 0.05 to 0.4%.

Słowa kluczowe

EN

addition; industrial feed mixture; 2-hydroxy-4-[methythio]butanoic acid; determination method; methionine hydroxyl

• Wydawca: -
• Czasopismo: Electronic Journal of Polish Agricultural Universities. Series Agricultural Engineering
• Rocznik: 2006
• Tom: 09
• Numer: 1
• Opis fizyczny: http://www.ejpau.media.pl/volume9/issue1/art-12.html

Twórcy

autor

Matyka S.

• Agaricultural University in Lublin, 48 Doswiadczalna Street, 20-236 Lublin, Poland

autor

Rubaj J.

autor

Korol W.

autor

Bielecka G.

Bibliografia

• Adisseo – informational materials, 2004. http://www.adisseonotrhamerica.com/RhodimetGuide/ug_88_analysis.asp.
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• Ontiveros R.R., Shermer W.D., Berner R.A., 1987. An HPLC method for the determination of 2-hydroxy-4-(methylthio)butanoic acid (HMB) in supplemented animal feeds. J. Agric. Food Chem 35, 5, 692-694.
• Wauters D., De Mol J., De Temmeraman L., 1990. Simultaneous liquid chromatographic determination of methionine hydroxy analogue and DL-methionine in feed formulations. J. of Chromatography 516, 375-382.