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1995 | 42 | 2 |

Tytuł artykułu

Nonhistone chromosomal proteins [NHCP] in adenocarcinoma of human endometrium

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Concentration and electrophoretic pattern of nonhistone chromosomal proteins (NHCP) in cancer and normal endometrium were examined. In normal tissues the NHCP/DNA ratio ranged from 0.6 to 0.8 whereas in malignant tissues from 1.2 to 1.7. Mean nuclear concentration of NHCP was 14.16 pg/cell nucleus of normal endometrium and 33.35 pg/cell nucleus of cancer tissue. The two tissues differed in the electrophoretic pattern. Heavy fractions (>60 kDa — 36%) predominated in normal tissue whereas light fractions (< 30 kDa — 48%) in malignant tissue samples. The percentage of intermediate fractions (30-60 kDa) was similar. Neoplastic endometrium shows, irrespective of the patient's age, a high NHCP content. It seems that NHCP, especially the fraction extracted with 0.35 M NaCl, because of its tissue and cell specificity are involved in neoplasm formation.

Wydawca

-

Rocznik

Tom

42

Numer

2

Opis fizyczny

p.191-195,fig.

Twórcy

autor
  • Medical Academy of Lublin, Jaczewskiego 8, 20-950 Lublin, Poland

Bibliografia

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  • 7. Lepp, W.A. & Martinez, P. (1989) Solid-phase enzyme immunoassay for the detection of HMG nonhistone proteins in their native structure. }. Immunoassay 10,449-465.
  • 8. Wen, B.G. & Chiu, J.F. (1987) Monoclonal antibodies to nonhistone proteins associated with human colon cancer nuclear matrix. Int. J. Biochem. 19,321-328.
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  • 13. Miturski, R. (1992) Immunological differences of nonhistone chromosomal proteins (NHCP) of myometrium and myomas in women. Gin. Pol 63,227-231 (in Polish).
  • 14. Bonner, J., Chalkley, G.R., Dahmus, M., Fambrough, D., Fujimura, F., Huang, R.C., Huberman, J., Jensen, R., Marushige, K., Ohlenbush, H., Oliviera, B. & Widholm, J. (1968) Isolation and characterisation of chromosomal nucleoproteins; in Methods Enzymol. 12B, 3-18.
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  • 24. Hnilica, L.S., Schmidt, W.N., Stryjecka-Zimmer, M., Duhl, D.M. & Briggs, R.C. (1983) Antibodies to chromosomal non-histone proteins in malignant cells; in Biochemical and biological markers of neoplastic transformation (Chandra,P., ed.) pp. 277-294, Plenum Publ. Corp.
  • 25. Miturski, R. (1993) Nonhistone chromosomal proteins in women uterus endometrial cancer. (PhD Thesis) Med. Acad. Lublin (in Polish).
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Typ dokumentu

Bibliografia

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bwmeta1.element.agro-article-84239b94-3869-48ca-812e-e23d06c0cf1a
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