Interaction of three Caenorhabditis elegans isoforms of translation initiation factor eIF4E with mono- and trimethylated mRNA 5' cap analogues

• Autorzy: Stachelska A. , Wieczorek Z. , Ruszczynska K. , Stolarski R. , Pietrzak M. , Lamphear B.J. , Rhoads R.E. , Darzynkiewicz E. , Jankowska-Anyszka M.
• Języki publikacji: EN

Abstrakty

EN

Translation initiation factor eIF4E binds the m G7 cap of eukaryotic mRNAs and me­diates recruitment of mRNA to the ribosome during cap-dependent translation initia­tion. This event is the rate-limiting step of translation and a major target for translational control. In the nematode Caenorhabditis elegans, about 70% of genes express mRNAs with an unusual cap structure containing m3 2,2,7 G, which is poorly rec­ognized by mammalian eIF4E. C. elegans expresses five isoforms of eIF4E (IFE-1, IFE-2, etc.). Three of these (IFE-3, IFE-4 and IFE-5) were investigated by means of spectroscopy and structural modelling based on mouse eIF4E bound to m7GDP. Intrinsic fluorescence quenching of Trp residues in the IFEs by iodide ions indicated structural differences between the apo and m7G cap bound proteins. Fluorescence quenching by selected cap analogues showed that only IFE-5 forms specific complexes with both m7G- and m3 2,2,7 G-containing caps (Kas 2 x 106M–1 to 7 x 106M–1) whereas IFE-3 and IFE-4 discriminated strongly in favor of m7G-containing caps. These spectroscopic results quantitatively confirm earlier qualitative data derived from affinity chromatography. The dependence of Kas on pH indicated optimal cap binding of IFE-3, IFE-4 and IFE-5 at pH 7.2, lower by 0.4 pH units than that of eIF4E from human erythrocytes. These results provide insight into the molecular mechanism of recognition of structurally different caps by the highly homologous IFEs.

Słowa kluczowe

EN

fluorescence; Caenorhabditis elegans; eukaryotic initiation factor; translation initiation; mRNA; isoform; cap structure; translation initiation factor

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2002
• Tom: 49
• Numer: 3
• Opis fizyczny: p.671-682,fig.

Twórcy

autor

Stachelska A.

• University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

autor

Wieczorek Z.

autor

Ruszczynska K.

autor

Stolarski R.

autor

Pietrzak M.

autor

Lamphear B.J.

autor

Rhoads R.E.

autor

Darzynkiewicz E.

autor

Jankowska-Anyszka M.

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