EN
The purpose of the studies was to make a thorough examination of interaction between ascorbic acid and manganese in realtion to their absorption from the alimentary tract of hens. This seems to be a new issue and there are no publications on the subject in the literature available. The research has presented the influence of magnesium on the absorption of vitamin C and, vice versa, the influence of ascorbic acid on the absorption of magnesium by the method of perfused intestinal loop „in vivo” according to Mykkanen and Nys. Manganese radically increased the absorption of ascorbic acid from jejunum and caecum of chickens. The inversely proportional dependence between the concentration of mangenese in the solution and the amount of vitamin C absorbed has been proved. A substantial increase of absorption of vitamin C in the jejunum from 2.60 mg/ /l/cm²/60 min to 4.51 mg/l/cm²/60 min and in the caecum from 4.10 mg/l/cm²/60 min to 7.41 mg/l/cm²/60 min was determined. Ascorbic acid, however, decreased the absortion of manganese. Vitamin C decreased the absorption of manganese in the jejunum from 1.08 mg/l/cm²/60 min to 0.63 mg/l/cm² 60 min and in the caecum from 2.69 mg/l/cm²/60 min to 0.92 mg/l/cm²/60 min. Ascorbic acid in perfused fluid was marked by the Roe-Kuether method. In time, along with the flow of perfused fluid, the conditions of absorption changed: pH increased and the amount of vitamin C and manganase absorbed by intestine was decreased. This phenomenon is caused by a natural process connected with the saturation of the intestine wall with the vitamin concentrated during the earlier intense absorption, the increased level of ascorbic acid in the blood, as well as the secretion into the light of the jejunum enzymes, electrolites and mucin. The results can be useful for nutrition, preventative care and medical therapy of domestic animals and people.