Presence of Shiga toxin-producing Escherichia coli 0157:H7 and the Stx2 gene and antibody response to Shiga toxin 2 in dairy cattle in Chongqing, People Republic of China

• Autorzy: Ding H , Wang H. , Mao X. , Zou Q.
• Języki publikacji: EN

Abstrakty

EN

This study intended to acquire the data on Escherichia coli O157:H7 isolated from dairy cattle, heifers, and calves, and of Stx2 expressed in STEC in Chongqing. Three hundred cattle rectal faecal samples were collected and E. coli O157:H7 was identified by the isolation of the bacteria, serological tests, and PCR. Moreover, the stx2 gene's genome was examined. Three hundred and twenty six milk samples were tested for the presence of immunoglobulin G antibodies to Stx2. Six E. coli O157:H7 isolates, which did not carry stx2 were obtained from the faecal samples. Forty seven positive Stx2 antibody samples were obtained in the milk samples, whereas the Stx2 antibody was not detected in the farm where the E. coli O157:H7 isolates were obtained. The prevalence of O157:H7 is lower than the prevalence of the Stx2 antibody, and the isolates lack stx2. However, it is possible that the non-O157 STEC transfers the stx2 gene to O157:H7 horizontally, and makes O157:H7 become more virulent.

Słowa kluczowe

EN

cattle; Escherichia coli; isolation; identification; polymerase chain reaction; ELISA test; China; Shiga toxin; dairy cattle; Chongqing

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2009
• Tom: 53
• Numer: 2
• Opis fizyczny: p.205-208,ref.

Twórcy

autor

Ding H

• Third Military Medical University, Chongqing, 400038, China

autor

Wang H.

autor

Mao X.

autor

Zou Q.

Bibliografia

• 1. Baets L.D., Taelen I.V., Filette M.D., Piérard D., Allison L., Greve H.D., Hernalsteens J-P., Imberechts H.: Genetic typing of shiga toxin 2 variants of Escherichia coli by PCR-restriction fragment length polymorphism analysis. Appl Environ Microbiol 2004, 70, 6309-6314.
• 2. Beutin L., Kaulfuss S., Cheasty T., Brandenburg B.O., Zimmermann S., Gleier K., Willshaw G.A., Smith H.R.: Characteristics and association with disease of two major subclones of Shiga toxin (Verocytotoxin)-producing strains of Escherichia coli (STEC) O157 that are present among isolates from patients in Germany. Diagn Microbiol Infect Dis 2002, 44, 337-346.
• 3. Crump J.A., Sulka A.C., Langer A.J., Schaben C., Crielly A.S., Gage R., Baysinger M., Moll M., Withers G., Toney D.M., Hunter S.B., Hoekstra R.M., Wong S.K., Griffin P.M., Van Gilder T.J.: An outbreak of Escherichia coli O157:H7 infections among visitors to a dairy farm. N Engl J Med 2002, 347, 555-560.
• 4. Desmarchelier P.M., Bilge S.S., Fegan N., Mills L., Vary Jr J.C., Tarr P.I.: A PCR specific for Escherichia coli O157 based on the rfb locus encoding O157 lipopolysaccharide. J Clin Microbiol 1998, 36, 1801-1804.
• 5. Gannon V.P., D'Souza S., Graham T., King R.K., Rahn K., Read S.: Use of the flagellar H7 gene as a target in multiplex PCR assays and improved specificity in identification of enterohemorrhagic Escherichia coli strains. J Clin Microbiol 1997, 35, 656-662.
• 6. Griffin P.M., Tauxe R.V.: The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome. Epidemiolc Rev 1991, 13, 60-98.
• 7. Guo X., Shi Z., Gu L., Zhuang L., Pan H.: Using multiplex PCR for the detection of virulence genes in Escherichia coli O157:H7. Zhonghua Liu Xing Bing Xue Za Zhi 2000, 21, 410-412.
• 8. Heuvelink A.E., Van de Kar N.C.A.J., Meis J.F.G.M., Monnens L.A.H., Melchers W.J.G.: Characterization of verocytotoxin-producing Escherichia coli O157 isolates from patients with haemolytic uraemic syndrome in Western Europe. Epidemiol Infect 1995, 115, 1-14.
• 9. Karch H., Russmann H., Schmidt H., Schwarzkopf A., Heesemann J.: Long-term shedding and clonal turnover of enterohemorrhagic Escherichia coli O157 in diarrheal diseases. J Clin Microbiol 1995, 33, 1602-1605.
• 10. Karmali M.A.: Infection by verocytotoxin-producing Escherichia coli. Clin Microbiol Rev 1989, 2, 15-38.
• 11. Karmali M.A., Steele B.T., Petric M., Lim C.: Sporadic cases of hemolytic uremic syndrome associated with fecal cytotoxin and cytotoxin-producing Escherichia coli. Lancet 1983, 1, 619-620.
• 12. Ludwig K., Karmali M.A., Sarkim V., Bobrowski C., Petric M., Karch H., Müller-Wiefel D.E. and Arbeitsgemeinschaft für Pädiatrische Nephrologie.: Antibody response to shiga toxins stx2 and stx1 in children with enteropathic hemolytic-uremic syndrome. J Clin Microbiol 2001, 39, 2272-2279.
• 13. Ludwig K., Muller-Wiefel D.E.: Pathomechanisms in the haemolytic-uraemic syndrome. Nephrol Dial Transplant 1998, 13, 23-27.
• 14. Navarro A., Eslava C., Hernandez U., Navarro-Henze J.L., Aviles M., Torre G.G., Cravioto A.: Antibody responses to Escherichia coli O157 and other lipopolysaccharides in healthy children and adults. Clin Diagn Lab Immunol 2003, 10, 797-801.
• 15. Ni D., Wang H., Gu L., Guo X., Zhuang L., Shi P., Pan H., Shi Z., Hu X., Liu, G.: Surveillance of Escherichia coli O157:H7 among animals in Jiangsu province in 1999. Zhonghua Liu Xing Bing Xue Za Zhi 2002, 23, 102-104.
• 16. Nishikawa Y., Zhou Z., Hase A., Ogasawara J., Cheasty T., Haruki K.: Relationship of genetic type of shiga toxin to manifestation of bloody diarrhea due to enterohemorrhagic Escherichia coli serogroup O157 isolates in Oaka city, Japan. J Clin Microbiol 2000, 38, 2440-2442.
• 17. Reymond D., Johnson R.P., Karmali M.A., Petric M., Winkler M., Johnson S., Rahn K., Renwick S., Wilson J., Clarke R.C., Spika J.: Neutralizing antibodies to Escherichia coli vero cytotoxin 1 and antibodies to O157 lipopolysaccharide in healthy farm family members and urban residents. J Clin Microbiol 1996, 34, 2053-2057.
• 18. Riley L.W., Remis R.S., Helgerson S.D., McGee H.B., Wells J.G., Davis B.R., Hebert R.J., Olcott E.S., Johnson L.M., Hargrett N.T., Blake P.A., Cohen M.L.: Hemorrhagic colitis associated with a rare Escherichia coli serotype. N Engl J Med 1983, 308, 681-685.
• 19. Vali L., Wisely K.A., Pearce M.C., Turner E.J., Knight H.I., Smith A.W., Amyes S.G.B.: High-level genotypie variation and antibiotic sensitivity among Escherichia coli O157 strains isolated from two Scottish beef cattle farms. Appl Environ Microbiol 2004, 70, 5947-5954.
• 20. Zadik P.M., Chapman P.A., Siddons C.A.: Use of tellurite for the selection of verocytotoxigenic Escherichia coli O157. J Med Microbiol 1993, 39, 155-158.