Zmiany receptorow steroidowych macicy po antykoncepcji estrogenowej u suk

• Autorzy: Jurka P , Snochowski M. , Boryczko Z.

Warianty tytułu

EN

Changes in uterine steroid receptors after estrogen contraception in bitches

• Języki publikacji: PL

Abstrakty

EN

The aim of the present study was to evaluate the effect of estradiol benzoate (EB) treatment on estradiol (E2) and progesterone (P4) in blood, and on estrogen (ER) and progesterone (PR) receptors in the uteri of mixed breed bitches. Injections of 10 µg EB/kg (Mesalin, Intervet) and 100 µg EB/kg (Oestradiolum benzoicum, Polfa) were given on day 3 and 5 after the first acceptance of males. Steroids were measured in samples collected from the vena cephalic using the immunofluorescent method. The average level of E2 was elevated to 190 and 260 pg/ml, respectively, and declined to control values within less than 12 days. An increase in P4 was observed only during the first 4 days. ER and PR were determined in uterine samples collected during hysterectomy performed on day 14 or 28 following the first EB injection. The radioligand assay was used for soluble (100.000 g cytosol) and structural (0.6 M NaCl extract) fractions. Typical binding characteristics for ER and PR were found in all analyzed samples. The amount of ER was unchanged over time in the untreated group, while PR significantly declined. The lower dose caused a 3-fold elevation of ER and PR (mainly soluble fractions) which on day 28 declined to control values with the exception of an uncompleted decline of soluble ER. The effect of higher doses on ER was only significant in its soluble fraction on day 14. However its influence on the PR (initially unchanged) occurred on day 28 and was elevated more than 10-times (mainly structural fraction). The results obtained indicate that uterine ER and PR responded differently to high and low doses of estrogen treatment.

Słowa kluczowe

PL

receptory estrogenowe; receptory progesteronowe; psy; srodki antykoncepcyjne; zmiany; macica; suki; benzoesan estradiolu; antykoncepcja hormonalna

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2007
• Tom: 63
• Numer: 09
• Opis fizyczny: s.1095-1099,rys.,tab.,bibliogr.

Twórcy

autor

Jurka P

• Szkola Glowna Gospodarstwa Wiejskiego, ul.Nowoursynowska 159 C, 02-766 Warszawa

autor

Snochowski M.

autor

Boryczko Z.

Bibliografia

• 1.Boryczko Z., Katkiewicz M., Bostedt H., Gajewski Z.: Ropomacicze u suk etiopatogeneza, objawy, rozpoznawanie i leczenie. Medycyna Wet. 2001, 57, 246- -250.
• 2.Botos J., Xian W., Smith D. F., Smith C. L.: Progesterone receptor deficient in chromatin binding has an altered cellular state. J. Biol. Chem. 2004, 279, 15231- -15239.
• 3.Bowen R. A., Olson P. N., Behrendt M. D., Wheeler S. L., Husted W., Nett T. M.: Efficacy and toxicity of estrogens commonly used to terminate canine pregnancy. J. Am. Vet. Med. Assoc. 1985, 186, 783-788.
• 4.Concannon P. W., McCann J. P., Temple M.: Biology and endocrinology of ovulation, pregnancy and parturition in the dog. J. Reprod. Fertil. Suppl. 1989, 39, 3-25.
• 5.Concannon P. W., Meyers-Wallen V. N.: Current and proposed methods for contraception and termination of pregnancy in dogs and cats. J. Am. Vet. Med. Assoc. 1991, 198, 1214-1225.
• 6.Edqvist L. E., Johansson E. D., Kasstrom H., Olsson S. E., Richkind M.: Blood plasma levels of progesterone and oestradiol in the dog during the oestrous cycle and pregnancy. Acta Endocrinol. (Copenh) 1975, 78, 554-564.
• 7.Eilts B. E.: Pregnancy termination in the bitch and queen. Clin. Tech. Small Anim. Pract. 2002, 17, 116-123.
• 8.England G., Concannon P. W.: Determination of the optimal breeding time in the bitch: Basic considerations, [w:] Concannon P. W., England G., Verstegen III J., Linde-Forsberg C. (wyd.): Recent Advances in Small Animal Reproduction. International Veterinary Information Service, Ithaca NY 2002.
• 9.Fernandes P. A., Bowen R. A., Sawyer H. R., Nett T. M., Gorell T. A.: Concentration of receptors for estradiol and progesterone in canine endometrium during estrus and diestrus. Am. J. Vet. Res. 1989, 50, 64-67.
• 10.Gadducci A., Biglia N., Sismondi P., Genazzani A. R.: Breast cancer and sex steroids: Critical review of epidemiological, experimental and clinical investigations on etiopathogenesis, chemoprevention and endocrine treatment of breast cancer. Gynecol. Endocrinol. 2005, 20, 343-360.
• 11.Grümmer R., Hewitt S. W., Traub O., Korach K. S., Winterhager E.: Different regulatory pathways of endometrial connexin expression: preimplantation hormonal-mediated pathway versus embryo implantation-initiated pathway. Biol. Reprod. 2004, 71, 273-281.
• 12. Johnston S. D., Kiang D. T., Seguin B. E., Hegstad R. L.: Cytoplasmic estrogen and progesterone receptors in canine endometrium during the estrous cycle. Am. J. Vet. Res. 1985, 46, 1653-1658.
• 13. Jurka P.: Ocena zmian zachodz¹cych w macicy i jajnikach po stosowaniu steroidowych preparatów antykoncepcyjnych u suk. Praca doktorska, SGGW, Warszawa 1999.
• 14. Jurka P., Snochowski M., Boryczko Z.: Wpływ dawki estrogenu na zmiany morfologiczne jajnika i macicy u suk. Medycyna Wet. 2007, w druku.
• 15.Karczewski W., Ostrzeszewicz G., Nagajewski M.: Analiza czynników usposabiających do występowania ropomacicza oraz wyników leczenia operacyjnego tego schorzenia Medycyna Wet. 1984, 4, 487-488.
• 16.Koehler K. F., Helguero L. A., Haldosén L. A., Warner M., Gustafsson J. A.: Reflections on the discovery and significance of estrogen receptor b. Endocr. Rev. 2005, 26, 465-478.
• 17.Kooistra H. S., den Hertog E., Okkens A. C., Mol J. A., Rijnberk A.: Pulsatile secretion pattern of growth hormone during the luteal phase and mid-anoestrus in beagle bitches. J. Reprod. Fertil. 2000, 119, 217-222.
• 18.Kurita T., Lee K. J., Saunders P. T. K., Cooke P. S., Taylor J. A., Lubahn D. B., Zhao C., Mäkelä S., Gustafsson J. A., Dahiya R., Cunha G. R.: Regulation of progesterone receptors and decidualization in uterine stroma of the estrogen receptor-a knockout mouse. Biol. Reprod. 2001, 64, 272-283.
• 19.Lessey B. A., Gorell T. A.: A cytoplasmic estradiol receptor in the immature Beagle uterus. J. Steroid. Biochem. 1980, 13, 211-217.
• 20.Levin E. R.: Integration of the extranuclear and nuclear actions of estrogen. Mol. Endocrinol. 2001, 19, 1951-1959.
• 21. Nuclear Receptors. Nomenclature Committee. Cell 1999, 97, 161-163.
• 22.Qiu M., Olsen A., Faivre E., Horwitz K. B., Lange C. A.: Mitogen-activated protein kinase regulates nuclear association of human pogesterone receptors. Mol. Endocrinol. 2003, 17, 628-642.
• 23.Reynaud K., Fontbonne A., Marseloo N., Thoumire S., Chebrout M., de Lesegno C. V., Chastant-Maillard S.: In vivo meiotic resumption, fertilization and early embryonic development in the bitch. Reproduction 2005, 130, 193-201.
• 24. Snochowski M.: Receptory tkankowe. Metody radioimmunologiczne i radiokompetycyjne stosowane w klinice (Red. Kokot F., Stupnicki R.) PZWL, Warszawa 1985, 3, 55-66.
• 25. Snochowski M., Romanowicz K.: The use of estrogen receptor for evaluation of phytoestrogens activities in mammals. Pol. J. Food Nutr. Sci. 2003, 51, 175-187.
• 26. Sutton D. J., Geary M. R., Bergman J. G.: Prevention of pregnancy in bitches following unwanted mating: a clinical trial using low dose oestradiol benzoate. J. Reprod. Fertil. Suppl. 1997, 51, 239-243.
• 27.Tata J. R.: One hundred years of hormones: A new name sparked multidisciplinary research in endocrinology, which shed light on chemical communication in multicellular organisms. EMBO Rep. 2005, 6, 490-496.
• 28.Tsutsui T., Mizutani W., Hori T., Oishi K., Sugi Y., Kawakami E.: Estradiol benzoate for preventing pregnancy in mismated dogs. Theriogenology 2006, 66, 1568- -1572.
• 29.Turgeon J. L., McDonnell D. P., Martin K. A., Wise P. M.: Hormone therapy: physiological complexity belies therapeutic simplicity. Science 2004, 304, 1269- -1273.
• 30.Tyagi R. K., Amazit L., Lescop P., Milgrom E., Guiochon-Mantel A.: Mechanisms of progesterone receptor export from nuclei: role of nuclear localization signal, nuclear export signal, and Ran guanosine triphosphate. Mol. Endocrinol. 1998, 12, 1684-1695.
• 31.Wanke M. M., Romagnoli S., Verstegen J., Concannon P. W.: Pharmacological approaches to pregnancy termination in dogs and cats including the use of prostaglandins, dopamine agonists, and dexamethasone, [w:] Concannon P. W., England G., Verstegen J., Linde-Forsberg C. (wyd.): Recent Advances in Small Animal Reproduction. Ithaca 2002.
• 32.Weilenmann R., Arnold S., Dobeli M., Rusch P., Zerobin K.: Estradiol and progesterone concentrations in the plasma of nonpregnant bitches during the sexual cycle. Schweiz Arch. Tierheilkd. 1993, 135, 51-57.
• 33.Weng B. C., Chew B. P., Wong T. S., Park J. S., Kim H. W., Lepine A. J.: Betacarotene uptake and changes in ovarian steroids and uterine proteins during the estrous cycle in the canine. J. Anim. Sci. 2000, 78, 1284-1290.