Dynamics of estrogen-induced oxidative stress

• Autorzy: Kobiela J. , Stefaniak T. , Krajewski J. , Kalinska-Blach B. , Zurawa-Janicka D. , Lachinski A. , Gackowski D. , Olinski R. , Nowak J. , Knap N. , Lipinska B. , Sledzinski Z. , Wozniak M.
• Języki publikacji: EN

Abstrakty

EN

The objective of this study was to assess the dynamics of oxidative damage to cellular macromolecules such as proteins, lipids and DNA under conditions of oxidative stress triggering early stages of estrogen-dependent carcinogenesis. A rodent model of carcinogenesis was used. Syrian hamsters were sacrificed after 1, 3, 5 h and one month from the initial implantation of estradiol. Matching control groups were used. Kidneys as target organs for estradiol-mediated oxidative stress were excised and homogenized for biochemical assays. Subcellular fractions were isolated. Carbonyl groups (as a marker of protein oxidation) and lipid hydroxyperoxides were assessed. DNA was isolated and 8-oxodGuo was assessed. Electron paramagnetic resonance spectroscopy was used to confirm the results for lipid peroxidation. Exposition to estradiol in the rodent model leads to damage of macromolecules of the cell, including proteins and DNA, but not lipids. Proteins appear to be the primary target of the damage but are closely followed by DNA. It has previously been speculated that protein peroxides can increase DNA modifications. This time sequence was observed in our study. Nevertheless, the direct relation between protein and DNA damage still remains unsolved.

Słowa kluczowe

EN

Syrian hamster; estrogen; carcinogenesis; oxidative stress; DNA damage; protein oxidation; rodent model; lipid peroxidation; breast cancer; estradiol

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2007
• Tom: 54
• Numer: 2
• Opis fizyczny: p.289-295,fig.,ref.

Twórcy

autor

Kobiela J.

• Medical University of Gdansk, Debinki 1, 80-211 Gdansk, Poland

autor

Stefaniak T.

autor

Krajewski J.

autor

Kalinska-Blach B.

autor

Zurawa-Janicka D.

autor

Lachinski A.

autor

Gackowski D.

autor

Olinski R.

autor

Nowak J.

autor

Knap N.

autor

Lipinska B.

autor

Sledzinski Z.

autor

Wozniak M.

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