Light activation of phospholipase A2 in the photoreceptor of the crayfish [Procambarus clarkii]

• Autorzy: Kashiwagi T , Meyer-Rochow V.B. , Nishimura K. , Eguchi E.
• Języki publikacji: EN

Abstrakty

EN

Retinal lipids of crayfish, kept at 4°C under continuous darkness for 3 weeks, consisted mainly of phosphatidylcholine (PC) and phosphatidylethanolamine (PE); sphingomyelin (SM), phosphatidylinositol (PI) and phosphatidylserine (PS) were minor contributors. PI, involved in the phototransduction cascade, never reached greater concentrations than 7% of the total. High concentrations of polyunsaturated fatty acids (PUFA) such as 20:4n-6, 20:5n-3 and 22:6n-3 (DHA, docosahexaenoic acid) were present in PC, PE and PS, but scarce in SM and PL In retinae of crayfish kept at 4°C in darkness for 3 weeks and then exposed to white light (6 h; ca. 4,500 lx), SM and PS remained seemingly unaffected. PC, however, significantly decreased within 10 min to 65% of the initial value and 50% at 180 min. To study the reduction of PC, lipids of retinae suspended in physiological solution with/without phospholipase C (PLC) and phospholipase A2 (PLA2) inhibitors such as DMDA (=DEDA), manoalide, ET-I8-OCH3, and U-73122 were measured. Only free fatty acids (FFA) of retinae with inhibitors of PLA2 like DMDA and manoalide decreased. Retinae irradiated by white light for 3 h displayed a significant reduction of PC, compared with those that had remained in continuous darkness. However, the PC of retinae with PLA2-inhibitors was not decreased by light. Our results provide evidence that not only photoreceptor cell PLC, but also PLA2 is activated by light.

Słowa kluczowe

EN

crayfish; mitochondrial membrane; Procambarus clarkii; fatty acid composition; phospholipid; plasma membrane; vision; endoplasmic reticulum; phospholipase A2; compound eye; retinal lipid; gas chromatography; phototransduction; light; photoreceptor

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2000
• Tom: 60
• Numer: 1
• Opis fizyczny: p.9-16,fig.

Twórcy

autor

Kashiwagi T

• Kurume University, 67 Asahi-machi, Kurume, Fukuoka 830-0011, Japan

autor

Meyer-Rochow V.B.

autor

Nishimura K.

autor

Eguchi E.

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