Effect of anti-IL-5 antibody on the intestinal eosinophils and IgA plasma cells response in mice infected with Trichinella spiralis

• Autorzy: Doligalska M , Laskowska M , Sinski E
• Języki publikacji: EN

Abstrakty

EN

The effect of anti-IL-5 monoclonal antibodies (mAb) on the level of eosinophilia, the number of IgA plasma cells in lamina propria and the level of IL-5 in scraped mucosa of small intestine in BALB/c mice was examined. Eosinophils and plasma cells in lamina propria were assessed in frozen sections of small intestine. The level of IL-5 was monitored using capture ELISA. The influence of mAb anti-IL-5 on the total cell number and number of eosinophils was measured in the peritoneal cavity. Intraperitoneal injection of 0.5 mg anti-IL-5 mAb on the 3rd and 5th day after infection (DAI) with 200 T. spiralis larvae strongly depressed both eosinophils and IgA plasma cells response in the lamina propria on day 15th and 24th after infection. There was a positive correlation between the number of eosinophils and the level of IL-5 as well as between the number of IgA plasma cells and level of IL-5. IL-5 was essential for eosinophils and IgA response in small intestine and a partial role for IL-5 in protection against T. spiralis was observed.

Słowa kluczowe

EN

infection; mice; eosinophil; antibody; parasite; plasma cell; mouse; monoclonal antibody; lamina propria; nematode; parasitic infection; larva; Trichinella spiralis; muscle tissue; intestinal eosinophil

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 1997
• Tom: 42
• Numer: 3
• Opis fizyczny: p.176-179,fig.

Twórcy

autor

Doligalska M

• Department of Parasitology, Institute of Zoology, University of Warsaw, Krakowskie Przedmiescie 26-28, 00-927 Warsaw, Poland

autor

Laskowska M

• Department of Parasitology, Institute of Zoology, University of Warsaw, Krakowskie Przedmiescie 26-28, 00-927 Warsaw, Poland

autor

Sinski E

• Department of Parasitology, Institute of Zoology, University of Warsaw, Krakowskie Przedmiescie 26-28, 00-927 Warsaw, Poland

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