Effect of UVA and 8-methoxypsoralen, 4,6,4'-trimethylangelicin or chlorpromazine on apoptosis of lymphocytes and their recognition by monocytes

• Autorzy: Wolnicka-Glubisz A. , Fraczek J. , Skrzeczynska-Moncznik J. , Friedlein G. , Mikolajczyk T. , Sarna T. , Pryjma J.
• Języki publikacji: EN

Abstrakty

EN

Photopheresis (ECP) is an immunomodulatory therapy that involves extracorporeal exposure of isolated peripheral blood leukocytes to UVA irradiation in the presence of 8-methoxypsoralen (8-MOP) followed by their reinfusion to the patient. However, the underlying mechanism of ECP is not well understood yet. We selected 8-methoxypsoralen (8-MOP), chlorpromazine (CPZ) and 4,6,4'-trimethylangelicine (TMA) because of differences in their ability to induce immune suppression in rats in vivo. In this study, we investigated the role of UVA irradiation of lymphocytes in the presence of TMA, CPZ or 8-MOP on cell apoptosis, and their impact on adhesion of lymphocytes to monocytes in vitro. Apoptosis of lymphocytes and their sub-populations (lymphocytes T and B, NK cells) were determined by a flow cytometry, using AnnexinV-FITC, TUNEL assay and DNA content analysis and antibodies CD3, CD56, CD19. Mitochondrial potential was measured using CMXRos staining and the interaction of monocytes with lymphocytes was monitored by PKH26 Red Sigma staining of lymphocytes and subsequent use of flow cytometry. Our results show a significant increase of apoptosis of the photochemically treated lymphocytes and a decrease of their mitochondrial potential that depended on the dose and time after the treatment. Our data also reveal an increased recognition of apoptotic lymphocytes by freshly isolated monocytes.

Słowa kluczowe

EN

photopheresis; immunomodulatory therapy; extracorporeal exposure; peripheral blood leucocyte; ultraviolet irradiation; 8-methoxypsoralen; chlorpromazine; 4,6,4'-trimethylangelicin; apoptosis; monocyte; lymphocyte

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2010
• Tom: 61
• Numer: 1
• Opis fizyczny: p.107-114,fig.,ref.

Twórcy

autor

Wolnicka-Glubisz A.

• Jagiellonian University, Krakow, Poland

autor

Fraczek J.

autor

Skrzeczynska-Moncznik J.

autor

Friedlein G.

autor

Mikolajczyk T.

autor

Sarna T.

autor

Pryjma J.

Bibliografia

• Knobler R, Heald P. Extracorporeal photoimmunochemothe-rapy. In Dermatological Phototherapy and Photodiagnostic Methods, J. Kurtmann (ed). Berlin, Heidelberg, Springer-Verlag, 2001, pp. 248-260.
• Edelson RL. Cutaneous T cell lymphoma. The helping hand of dendritic cells. Ann NY Acad Sci 2001; 941: 1-11.
• Bethea D, Fullmer B, Syed S, et al. Psoralen photobiology and photochemotherapy: 50 years of science and medicine. J Dermatol Sci 1999; 19: 78-88.
• Duvic M, Hester JP, Lemak NA. Photopheresis therapy for cutaneous T-cell lymphoma. J Am Acad Dermatol 1996; 35: 573-579.
• Malawista SE, Trock DH, Edelson RL. Treatment of rheumatoid arthritis by extracorporeal photochemotherapy. Arthritis Rheum 1991; 34: 646-654.
• Meiser BM, Kur F, Reichenspurner H, et al. Reduction of the incidence of rejection by adjunct immunosuppresion with phototherapy after heart transplantation. Transplantation 1994; 57: 563-586.
• Yoo EK, Rook AH, Elenitsas R, Gasparro FP, Vowels BR. Apoptosis induction by ultraviolet light A and photochemotherapy in cutaneuous T-cell lymphoma: relevance to mechanism of therapeutic action. J Invest Dermatol 1996; 107: 235-242.
• Craciun LI, Stordeur P, Schandene L, et al. Increased production of interleukin-10 and interleukin-1 receptor antagonist after extracorporeal photochemotherapy in chronic graft-versus-host disease. Transplantation 2002; 74: 995-1000.
• Di Renzo M, Rubegni P, Pasqui AL, et al. Extracorporeal photopheresis affects interleukin (IL)-10 and IL-12 production by monocytes in patients with chronic graft-versus-host disease. Br J Dermatol 2005; 153: 59-65.
• Bladon J, Taylor PC. Extracorporeal photopheresis: a focus on apoptosis and cytokines. J Dermatol Sci 2006; 43: 85-94.
• Van Iperen HP, Beijersbergen van Henegouwen GJM. An aminal model and new photosensitizers for photopheresis. Photochem Photobiol 1993; 58: 571-574.
• Van Iperen HP, Brun BM, Caffieri S, Dall'Acqua F, Gasparro FP, Beijersbergen van Henegouwen GJM. The lack of efficacy of 4,6,4'-trimethylangelicin to induce immune suppression in an animal model for photopheresis: a comparison with 8-MOP. J Photochem Photobiol B 1996; 63: 577-582.
• Van Iperen HP, Beijersbergen van Henegouwen GJM. Chlorpromazine; a candidate drug for photopheresis. J Photochem Photobiol B 1996; 34: 217-224.
• Wolnicka A, Sarna T, Knobler R, Trautinger F. Differential effect of 8-methoxypsoralen, 4,6,4'-trimethylangelicine, and chlorpromazine on cell death and TNF-alpha production. Arch Dermatol Res 2002; 294: 147-151.
• Wolnicka-Glubisz A, Rajwa B, Dobrucki J, Skrzeczynska-Moncznik J, Beijersbergen van Henegouwen G, Sarna T. Phototoxicity, distribution and kinetics of association of UVA-activated chlorpromazine, 8-methoxypsoralen, and 4,6,4'-trimethylangelicin in Jurkat cells. J Photochem Photobiol B 2005; 78: 155-164.
• Wolnicka-Glubisz A, Rijnkels JM, Sarna T, Beijersbergen van Henegouwen GMJ. Apoptosis in leukocytes induced by UVA in the presence of 8-methoxypsoralen, chlorpromazine or 4,6,4'-trimethylangelicine. J Photochem Photobiol B 2002; 68: 65-72.
• Viola G, Fortunato E, Cecconet L, Disaro S, Basso G. Induction of apoptosis in Jurkat cells by photoexcited psoralen derivatives: Implication of mitochondrial dysfunctions and caspases activation. Toxicol in vitro 2007; 21: 211-216.
• Viola G, Fortunato E, Cecconet L, Del Giudice L, Dall'Acqua F, Basso G. Central role of mitochondria and p53 in PUVA-induced apoptosis in human keratinocytes cell line NCTC-2544. Toxicol Appl Pharmacol 2008; 227: 84-96.
• Bzowska M, Guzik K, Barczyk K, Ernst M, Flad H-D, Pryjma J. Increased IL-10 production during spontaneous apoptosis of monocytes. Eur J Immunol 2002; 32: 2011-2020.
• Byrne A, Reen DJ. Lypopolisaccharide induces rapid production of IL-10 by monocytes in the presence of apoptotic neutrophils. J Immunol 2002; 168: 1968-1977.
• Guzik K, Bzowska M, Dobrucki, Pryjma J. Heat-shocked monocytes are resistant to Staphylococcus aureus - induced apoptotic DNA fragmentation due to expression of HSP72. Infect Immun 1999; 67: 4216-4222.
• Janczyk A. Wolnicka-Glubisz A, Chmura A, et al. NO dependent phototoxicity of Roussin's black salt against cancer cells. Nitric Oxide 2004; 10: 42-50.
• Macho A, Decaudin D, Castedo M, et al. Chloromethyl-X-Rosamine is an aldehyde-fixable potential-sensitive fluorochrome for the detection of early apoptosis. Cytometry 1996; 25: 333-340.
• Li H, Zhu H, Xu CJ, Yuan J. Cleavage of BID by caspase 8 mediates the mitochondria damage in Fas pathway of apoptosis. Cell 1998; 94: 491-501.
• Horan PK, Slezak, SE. Stable cell membrane labelling. Nature 1989; 340: 167-168.
• Mikolajczyk T, Skrzeczynska-Moncznik J, Zarebski M, et al. Interaction of human peripheral monocytes with apoptotic polymorphonuclear cells. Immunology 2009; 128: 103-113.
• Weglarczyk K, Baran J, Zembala M, Pryjma J. Caspase-8 activation precedes alterations of mitochondrial membrane potential during monocyte apoptosis induced by phagocytosis and killing of Staphylococcus aureus. Infect Immun 2004; 72: 2590-2597.
• Enomoto DN, Schellekens PT, Yong SL, ten Berge IJ, Mekkes JR, Bos JD. Extracorporeal photochemotherapy (photopheresis) induces apoptosis in lymphocytes: a possible mechanism of action of PUVA therapy. Photochem Photobiol 1997; 65: 177-180.
• Aringer M, Graninger WB, Smolen JS, et al. Photopheresis treatment enhances CD95 (Fas) expression in circulating lymphocytes of patients with systemic sclerosis and induces apoptosis. Br J Rheumatol 1997; 36: 1276-1282.
• Wolnicka-Glubisz A, Lukasik M, Pawlak A, Wielgus A, Niziolek-Kierecka M, Sarna T. Peroxidation of lipids in liposomal membranes of different composition photosensitized by chlorpromazine. Photochem Photobiol Sci 2009; 8: 241-247.
• Schoonderwoerd SA, Beijersbergen van Henegouwen GM, Persons CC. Photobinding of 8-methoxypsoralen, 4,6,4'-trimethylangelicin and chlorpromazine to Wistar rat epidermal biomolecules in vivo. J Photochem Photobiol B 1991; 10: 257-268.
• Weston CR, Davis RJ. The JNK signal transduction pathway. Curr Opin Cell Biol 2007; 19: 142-149.
• Cho SG, Choi EJ. Apoptotic signaling pathways: caspases and stress-activated protein kinases. J Biochem Mol Biol 2002; 35: 24-27.
• Tournier C, Hess P, Yang DD, et al. Requirement of JNK for stress-induced activation of the cytochrome c mediated death pathway. Science 2000; 288(5467): 870-874.
• Bhowmick R, Girotti AW. Signaling events in apoptotic photokilling of 5-aminolevulinic acid-treated tumor cells: inhibitory effects of nitric oxide. Free Radic Biol Med 2009: 47: 731-740.
• Hasnis E, Bar-Shai M, Burbea Z, Reznick AZ. Mechanisms underlying cigarette-smoke induced NF-kB activation in human lymphocytes: the role of reactive nitrogen species. J Physiol Pharmacol 2007: 58(Suppl 5): 275-287.
• Papa S, Zazzeroni F, Pham CG, Bubici C, Franzoso G. Linking JNK signaling to NF-kB: a key to survival. J Cell Sci 2004; 117: 5197-5208.
• Grata-Borkowska U, Steciwko A, Pokorski M, et al. Effects of neuraminidase on apoptosis of blood lymphocytes in rats with implanted Morris tumor. J Physiol Pharmacol 2007; 58(Suppl 5): 253-262.
• Wasik M, Gorska E, Modzelewska M, Nowicki K, Jakubczak B, Demkow U. The influence of low-power helium-neon laser irradiation on function of selected peripheral blood cells. J Physiol Pharmacol 2007; 58(Suppl 5): 729-737.
• Voll RE, Hermann M, Roth EA, Stach C, Kalden JR, Girkontaite I. Immunosupresive effects of apoptotic cells. Nature 1997; 390: 350-351.
• Rivas JM, Ullrich SE. The role of IL-4, IL-10 and TNF-a in the immune supression induced by ultraviolet radiation. J Leukoc Biol 1994; 56: 769-775.
• Kehren J, Desvignes C, Krasteva M, et al. Cytotoxicity is mandatory for CD8(+) T cell - mediated contact hypersensitivity. J Exp Med 1999; 189: 779-786.
• Akiba H, Kehren J, Ducluzeau MT, et al. Skin inflammation during contact hypersensitivity is mediated by early recruitment of CD8+ T cytotoxic 1 cells inducing keratinocyte apoptosis. J Immunol 2002; 168: 3079-3087.