Oxidative damage to plant DNA in relation to growth conditions

• Autorzy: Bialkowski K. , Olinski R.
• Języki publikacji: EN

Abstrakty

EN

In this study we investigated the level of 8-oxo-2'-deoxyguanosine (8-oxodG) in DNA of Cardamine pratensis plants subjected to different growth conditions trying to answer the question whether factors like light and water accessibility or low temperature may have an impact on the total DNA oxidative damage. The level of this modified nucleoside was determined using HPLC coupled to UV absorbance and electrochemical detection (HPLC-UV-EC). We did not observe any statistically significant differences in 8-oxodG level between DNA of etiolated and light exposed plants as well as between DNA of regularly watered and drought-subjected plants. In contrast, we have shown that chilling (1®C for 28 h) brings about the increase of 8-oxodG level in DNA.

Słowa kluczowe

EN

oxidative damage; growth condition; 8-oxo-2'-deoxyguanosine; oxidative stress; 8-hydroxyguanine; chilling; drought; free radical; DNA; Cardamine pratensis; plant

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1999
• Tom: 46
• Numer: 1
• Opis fizyczny: p.43-49,fig.

Twórcy

autor

Bialkowski K.

• University School of Medical Sciences, M. Karlowicza 24, 85-092 Bydgoszcz, Poland

autor

Olinski R.

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