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2008 | 17 | 6 |

Tytuł artykułu

Effects of dietary transgenic Bacillus thuringiensis maize Poland on hive worker honeybees

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
To assess potential impacts of transgenic insect-resistant (MON 863 x MON 810) Bt maize-pollen con­sumption on hive honeybees, two-, three-, four- and five-day-old workers were fed during five days with a mixture of honey and sugar (Control, Group I), honey with non-transgenic Limagrain maize pollen (Group II) and honey with Bacillus thuringiensis maize pollen (Group III). The consumption rate, weight of bees, pro­tein content, antioxidant enzyme activities and total antioxidant status were estimated. There were no signifi­cant differences in any of the above parameters among the groups of bees fed on the three diets. Only trehalose and maltose levels were higher in bees fed a pollen-free control diet compared to bees fed either transgenic or non-transgenic maize pollen. Our data indicate that the transgenic pollen had no adverse impact on studied markers of young hive honeybees.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

17

Numer

6

Opis fizyczny

p.957-961,ref.

Twórcy

autor
  • University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland
autor
autor
autor

Bibliografia

  • 1. SHARMA H.C., SHARMA K. K., SEETHARAMA N. ORTIZ R.O. Prospects for using transgenic resistance to insects in crop improvement. Electron. J. Biotechnol. [online] 3 (2), 21, 2000, http://www.ejb.org/content/vol3/issue2/full/3.
  • 2. DUAN J. J., MARVIER M., HUESING J., DIVELY G., HUANG Z. Y. A meta-analysis of effect of Bt crops on honey bees (Hymenoptera: Apidae). PLoS ONE 3 (1): e 1415. doc: 10.1371/journal.pone.0001415, 2008.
  • 3. BR0DSGAARD F., BR0DSGAARD C. J., HANSEN H., LOVEI G. L. Environmental risk assessment of transgenic products using honey bee (Apis mellifera) larvae. Apidologie 34, 139, 2003.
  • 4. LIU B., XU C., YAN F., GONG R. The impacts of the pollen of insectresistant transgenic cotton on honeybees. Biodiver. Conserv. 14, 3487, 2005.
  • 5. MALONE L. A., PHAM-DELEGUE M. H. Effects of transgenic products on honey bees (Apis mellifera) and bumble­bees (Bombus sp.) Apidologie 32, 287, 2001.
  • 6. MALONE L. A. Potential effects of GM crops on honeybee health. Bee World 85 29, 2004.
  • 7. MORSE R. A., CALDERONE N. W. The value of honey bees as pollinators of US crops in 2000. Bee Culture 128, 1, 2001.
  • 8. WILLIAMS I. H. The EU regulatory framework for GM 16. foods in relation to bee products. Bee World 83, 78, 2002.
  • 9. HANLEY A. V., HUANG Z. Y., PETT W. L. Effects of dietary transgenic Bt corn pollen on larvae of Apis mellifera 17 and Galleria mellonella. J. Apicul. Res. 42(4), 77, 2003.
  • 10. WINSTON M. L. The biology of the honeybee. Harvard University Press. 1987. 18.
  • 11. JAMES C. Global status of commercialized Biotech/GM crops. ISAAA Brief No. 37. Ithaca NY. 2007.
  • 12. BABENDREIER D., KALBERER N., ROMEIS J., FLURI 19 P., BIGLER F. Pollen consumption in honey bee larvae: a step forward in the risk assessment of transgenic plants. Apidologie 35, 293, 2004.
  • 13. MALONE L. A., BURGESS E. P. J., GATEHOUSE H. S., VOISEY CH, R., TREGIDGA E. L., PHILIP B. A. Effects 20 of ingestion of a Bacillus thuringiensis toxin and a trypsin inhibitor on honey bee flight activity and longevity. Apidologie 32, 57, 2001.
  • 14. LIPIŃSKI Z., ŻÓŁTOWSKA K. Preliminary evidence 21 associating oxidative stress in honey bee drone brood with Varroa destructor. J. Apicul. Res. 44 (3), 126, 2005.
  • 15. PANZENBOCK, U., CRAILSHEIM, K., Glycogen in hon- 22 eybee queens, workers and drones (Apis mellifera carnica Poll.). J. Insect Physiol. 43, 155, 1997.
  • 16. BEAUCHAMP C., FRIDOVICH I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44, 276, 1971.
  • 17. RICE-EVANS C. A., DIPLOCK A. T., SYMSON M. C. R. Techniques in free radical research. Elsevier, Amsterdam. 1991. CHANCE B., MAEHLY A. C. Assay of catalases and per- oxidases. In: Methods in Enzymology, v. 2, (S. P. Colowick, N. O. Kaplan, Eds) Acad. Press, NY, 764, 1955.
  • 18. RE R., PELLEGRINI N., PROTEGGENTE A., PAN- NALA A., YANG M., RICE-EVANS C. Antioxidant activity applying and improved ABTS radical cation decolorization assay. Free Radic. Biol. Med., 26, 1231, 1999.
  • 19. BRADFORD M. M. A rapid and sensitive method for quantitation of microgram quanties of protein utilising the principle of protein-dye binding. Anal. Biochem. 72, 248, 1976.
  • 20. SCHUR A., TORNIERI I., NEUMANN C. Bt-Mais und Not- Bt-Mais: vergleichende Untersuchungen an Honigbienen (Tunnelzeltversuch) Apidologie 31, 616, 2000.
  • 21. ARPAIA S. Ecological impacts of Bt-transgenic plants: 1. Assessing possible effects of CryIIIB on honey bee (Apis mellifera L.) colonies. J. Genetic. Breed. 50, 315, 1996.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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