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2006 | 62 | 01 |

Tytuł artykułu

Wplyw chimeryzmu na wybrane cechy reprodukcyjne kogutow

Warianty tytułu

Języki publikacji

PL

Abstrakty

EN
Our objective was the identification of chimerism on the basis of some traits and the estimation of their effect on reproductive features. The investigation was conducted on 11 cocks of Zk (donor) breed, 10 WI cocks (blastoderm cells recipient) and 10 chimeric (Ch) cocks, raised and housed in the same environmental conditions. Chimerism was identified on the basis of different criteria: down color, germline chimerism (backcrossing), and blood chromosome W identification (PCR). Using the MultiScan software the concentration of spermatozoa in sperm from cocks of 23, 28 and 34-weeks-of-age was determined. From the data presented in this manuscript, it is evident that the production of the chicken chimeras using the blastodermal cells is not a one-off event. On the contrary 90% (9/10) of investigated cocks were identified as chimera. Three chimeras produced the low spermatozoa sperm concentration (from 653.000 to 772.000 /ml), and two chimeras did not produce sperm. Among the above-mentioned cocks, some had underdeveloped testis and others showed chromosome W in their blood. On the other hand, three chimeras produced a high number of spermatozoa (from 2. 289.000 to 2. 681.000 / ml) and two of them were characterized by the highest spermatozoa production in comparison with all 31 investigated Wl, Zk and Ch cocks.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

62

Numer

01

Opis fizyczny

s.85-88,tab.,bibliogr.

Twórcy

autor
  • Instytut Zootechniki, ul. Krakowska 1, 32-083 Balice k.Krakowa

Bibliografia

  • 1.Abercombie M., Hickman M., Johnson M. L., Thain M.: Dictionary of Biology. Pengium Books Ltd, London 1992.
  • 2.Bednarczyk M.: Manipulacje komórkami embrionalnymi ptaków. Biotechnologia 2003, 60, 36-47.
  • 3.Bednarczyk M., Łakota P., Siwek M.: Improvement of hatchability of chicken eggs injected by blastoderm cells. Poultry Sci. 2000, 79, 1823-1828.
  • 4.Bednarczyk M., Łakota P., Słomski R., Pławski A., Lipinski D., Siemieniako D., Lisowski M., Czekalski P., Grajewski B., Dłużniewska P.: Reconstitution of a chicken breed by inter se mating of germline chimeric birds. Poultry Sci. 2002, 81, 1347-1353.
  • 5.Carsience R. S., Clark M. E., Gibbins V. A. M., Etches R. J.: Germline chimeric chickens from dispersed donor blastodermal cells and compromised recipient embryos. Development 1993, 117, 669-675.
  • 6.Ginsburg M.: Primordial germ cell development in avians. Poultry Sci. 1997, 76, 91-95.
  • 7.Jaszczak K., Parada R., Guszkiewicz A.: Cytogenetic study of some tissues and age-related changes in cell proportions in a goat-sheep chimera. Cyt. Cell Gen. 1999, 84, 55-57.
  • 8.Kagami H., Tagami T., Matsubara Y., Harami T., Hanada H., Maruyama K., Sakurai M., Kuwana T., Naito M.: The developmental origin of primordial germ cells and the transmission of the donor-derived gametes in mixed-sex germline chimeras to the offspring in the chicken. Mol. Reprod. Dev. 1997, 48, 501-510.
  • 9.Kino K. B., Pain B., Leibo M., Cochrans M., Clark M. E., Etches R. J.: Production of chicken chimeras from injection of frozen-thawed blastodermal cells. Poultry Sci. 1997, 76, 753-760.
  • 10.Mozdziak P. E., Petitte J. N.: Status of transgenic chicken models for developmental biology. Dev. Dynamics 2004, 229, 414-421.
  • 11.Petitte J., Clark M. E., Liu G., Gibbins V. A. M., Etches R. J.: Production of somatic or germline chimeras in the chicken by transfer of early blastodermal cells. Development 1990, 108, 185-189.
  • 12.Petitte J., Liu G., Yang Z.: Avian pluripotent stem cells. Mechanisms Dev. 2004, 121, 1159-1168.
  • 13.Rui R., Shim H., Moyer A. L., Anderson D. L., Penedo C. T., Rowe J. D., BonDurant R. H., Anderson G. B.: Attempts to enhance production of porcine chimeras from germ cells and preimplantation embryos. Theriogenology 2004, 61, 1225-1235.
  • 14.Simkiss K., Luke G., Behnam J.: Female chromosomes in cockerel ejaculates. Proc. R. Soc. Lond. B. Biol. Sci. 1996, 263, 1245-1249.
  • 15.Thoraval P., Lasserre L., Coudert F., Dambrine G.: Production of germline chimeras obtained from Brown and White Leghorns by transfer of early blastodermal cells. Poultry Sci. 1994, 73, 1897-1905.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-6dfb9cec-9234-4b87-a968-0b8a108cf2be
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