Papaverine should not be used as an indicator for controlling the ability of the gastrointestinal tract muscle to relax in vitro

• Autorzy: Chlopecka M , Dziekan N. , Bakala A. , Wiechetek M. , Mendel M.
• Języki publikacji: EN

Abstrakty

EN

The aim of this research was to study the possibilities of using papaverine as a reference substance to control the ability of isolated gastrointestinal (GI) tract strips to relax. The effects of papaverine hydrochloride (0.001-100 µM) dissolved in distilled water, or in DMSO (0.5%), on the mechanical activity of isolated rat GI strips (stomach fundus and corpus, duodenum and jejunum) were studied. The obtained results show that papaverine provoked various responses of the examined muscle strips dependent on the part of GI tract and papaverine solvents used (water or DMSO). Papaverine applied as water solution caused muscle relaxation of all investigated gastrointestinal strips: the lowest effective (induced relaxation) concentration of papaverine was 10 µM for gastric corpus, jejunum and duodenum and 100 µM for gastric fundus. However, there was no dependence between the concentration of papaverine and the degree of muscle relaxation of the studied GI strips. Moreover, in case of gastric fundus strips, papaverine applied as 0.5% DMSO solution provoked different muscle responses: in the presence of 0.1 and 1 µM papaverine contraction occurred; administering papaverine at higher concentrations (10 and 100 µM) resulted in relaxation. The obtained results clearly indicate that papaverine does not fulfil the criteria set for the reference substance and should not be used as an indicator for controlling of gastrointestinal tract muscle relaxation in vitro.

Słowa kluczowe

EN

gastrointestinal tract muscle; relaxation; papaverine; ability; controlling; stomach corpus; stomach fundus; jejunum; duodenum; smooth muscle; mechanical activity

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2007
• Tom: 63
• Numer: 11 Supl.
• Opis fizyczny: p.1437-1439,fig.,ref.

Twórcy

autor

Chlopecka M

• Warsaw Agricultural University, Ciszewskiego 8, 02-786 Warsaw, Poland

autor

Dziekan N.

autor

Bakala A.

autor

Wiechetek M.

autor

Mendel M.

Bibliografia

• 1. 2000 Report of the AVMA Panel on Euthanasia. J. Am. Vet. Med. Assoc. 2001, 218, 669-696.
• 2. Baccari M. C., Bani D., Bigazzi M., Calamai F.: Influence of relaxin on the neurally induced relaxant responses of the mouse gastric fundus. Biol. Reprod. 2004, 71, 1325-1329.
• 3. Ceregrzyn M., Ozaki T., Kuwahara A., Wiechetek M.: 1: Sodium nitrite - a potent relaxant of rat stomach fundus: in vitro evidence. Can. J. Physiol. Pharmacol. 1998, 76, 9-12.
• 4. Danneman P. J., Stein S., Walshaw S. O.: Humane and practical implications of using carbon dioxide mixed with oxygen for anesthesia or euthanasia of rats. Lab. Anim. Sci. 1997, 47, 376-385.
• 5. Etus V., Tugay M., Utkan T., Muezzinoglu B., Ceylan S.: Foregut smooth muscle reactivity changes in the hydrocephalus-induced infantile rats. J. Surg. Res. 2004, 118, 197-202.
• 6. Fujimoto S., Mori M.: Characterization of capsaicin-induced, capsazepine-insensitive relaxation of ileal smooth muscle of rats. Eur. J. Pharmacol. 2004, 487, 175-182.
• 7. Harrison W. E., Mellor A. J., Clark J., Singer D. R. J.: Vasodilator pre-treatment of human radial arteries; comparison of effects of phenoxybenzamine vs papaverine on norepinephrine-induced contraction in vitro. Eur. Heart J. 2001, 22, 2209-2216.
• 8. Hazelhoff B., Malingre Th. M., Meijer D. K. F.: Antispasmodic Effects of Valeriana Compounds: an in vivo and in vitro Study on the Guinea-Pig Ileum, Arch Int. Pharmacodyn. 1982, 257, 274-287.
• 9. Huddart H., Saad K. H.: Papaverine-induced inhibition of electrical and mechanical activity and calcium movements of rat ileal smooth muscle. J. Exp. Biol. 1980, 86, 99-114.
• 10. Kaneda T., Shimizu K., Nakajyo S., Urakawa N.: The difference in the inhibitory mechanisms of papaverine on vascular and intestinal smooth muscles. Eur. J. Pharmacol. 1998, 55, 149-157.
• 11. Report of the International Consensus Meeting on Carbon Dioxide Euthanasia of Laboratory Animals, ANZCCART News 2006, 19.
• 12. Serajuddin A. T. M., Rosoff M.: pH-solubility profile of papaverine hydrochloride and its relationship to the dissolution rate of sustained-release pellets. J. Pharm. Sci. 1984, 73, 1203-1208.
• 13. Shimuzu K., Ichikawa T., Urakawa N., Nakajyo S.: Inhibitory mechanisms of papaverine on the smooth muscle of guinea pig urinary bladder. J. Pharmacol. 2000, 83, 143-149.
• 14. The Merck Index. An Encyclopedia of Chemicals, Drugs and Biologicals. 14th Edition. O'Neil M. J. (red.). Merck&Co., Inc., Whitehouse Station, N.J., USA 2006, 6973.
• 15. Trute A., Gross J., Mutschler E., Nahrstedt A.: In Vitro Antispasmodic Compounds of the Dry Extract Obtained from Hedera helix. Planta Med. 1997, 63, 125-129.
• 16. Tugay M., Utkan T., Utkan Z.: Effects of caustic lye injury to the esophageal smooth miscle reactivity: in vitro study. J. Surg. Res. 2003, 113, 128-132.