A simple method for the estimation of recombination frequencies and genetic distances

• Autorzy: Benito C , Gallego A.
• Języki publikacji: EN

Abstrakty

EN

We have developed an alternative approach to the maximum likelihood method for calculating recombination values and linkage intensities. This new method is simpler than those in current use in that it obviates the need for formulae and tables. Maximum likelihood methods imply the use of iterative procedures over highly complicated estimation equations (at least second degree polynomials), whereas simplified methods use single-step procedures involving simple linear equations. The proposed method uses the frequencies of the distinguishable phenotypes that came from the union of at least one recombinant gamete with another gamete. We performed Monte-Carlo simulations for various combinations of genetic distance and offspring size. The recombination values obtained using the new method were compared with those derived by the maximum likelihood method on both simulated and experimental data. They were found to be nearly identical. The observed distribution of the recombination frequency values does not differ significantly from the Normal distribution, except for extreme values of the mean, as the Skewedness and kurtosis coefficients do not differ from zero. Our method has a similar accuracy to the maximum likelihood methods for recombination frequencies smaller than 25 cM and the difference does not increase greatly for greater frequencies.

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2004
• Tom: 09
• Numer: 4A
• Opis fizyczny: p.617-634,fig.,ref.

Twórcy

autor

Benito C

• University Complutanse, 28040-Madrid, Spain

autor

Gallego A.

Bibliografia

• 1. Bateson, W. and Punnet, R.C. On gametic series involving reduplication of certain terms. J. Genet. 1 (1911) 293-302.
• 2. Collins, J.N. Gametic coupling as a cause of correlation. Am. Nat. 46 (1912) 569-590.
• 3. Emerson, R.A. The calculation of linkage intensities. Am. Nat. 50 (1916) 411-420.
• 4. Haldane, J.B.S. The probable errors of calculated linkage values, and the most accurate method for determining gametic from certain zygotic series. J. Genet. 8 (1919) 291-297.
• 5. Wellensiek, S.J. Methods for calculating the actual gametic F2 series from a given zygotic series. Genetica 9 (1927) 329-340.
• 6. Fisher, R.A. Statistical Methods for Research Workers (2nd Ed.) Oliver and Boyd, Edinburgh, (1928) pp 291-324.
• 7. Fisher, R.A. and Balmukan, B. The estimation of linkage from the offspring of selfed heterozygous. J. Genet. 20 (1928) 79-92.
• 8. Owen, F.V. Calculation of linkage intensities by product moment correlation. Genetics 13 (1928) 80-110.
• 9. Immer, F.R. Formulae and tables for calculating linkage intensities. Genetics 15 (1930) 81-89.
• 10. Stephens, W.L. Tables of the recombination fraction estimated from the product ratio. J. Genet. 39 (1939) 171-180.
• 11. Immer, F.R. and Henderson, M.T. Linkage studies in barley. Genetics 28 (1943) 419-440.
• 12. Allard, R.W. Formulas and tables to facilitate the calculation of recombination values in heredity. Hilgardia 24 (1956) 235-278.
• 13. Kuspira, J. and Bhambhani, R.N. A simple method for estimating recombination frequencies and linkage intensities from F2 data: examples from Triticum monococcum and other self-fertilizing diploid species. Theor. Appl. Genet. 68 (1984) 61-67.
• 14. Ritter, E., Gebhardt, C. and Salamini, F. Estimation of recombination frequencies and construction of RFLP linkage maps in plants from crosses between heterozygous parents. Genetics 125 (1990) 645-654.
• 15. Wehling, P. Inheritance linkage relationship and chromosomal localization of the glutamate oxaloacetate transaminase, acid phosphatase and diaphorase isozyme genes in Secale cereale L. Theor. Appl. Genet. 82 (1991) 569-576.
• 16. Lawrence, G.J. and Appels, R. Mapping the nucleolus organizer region, seed protein loci and isozyme loci on chromosome 1R in rye. Theor. Appl. Genet. 71 (1986) 742-749.
• 17. Devos, K.M., Atkinson, M.D., Chinoy, C.N., Liu, C.L. and Gale, M.D. RFLP-based genetic map of the homoeologous group 3 chromosome of wheat and rye. Theor. Appl. Genet. 83 (1992) 931-939.