Induction of heme oxygenase-1 and heat shock protein 70 in rat hepatocytes: the role of calcium signalling

• Autorzy: Silomon M , Bauer I. , Bauer M. , Nolting J. , Paxian M. , Rensing H.
• Języki publikacji: EN

Abstrakty

EN

Stress response genes including heat shock proteins are induced under a variety of conditions to confer cellular protection. This study investigated the role of calcium signaling in the induction of two stress response genes, heme oxygenase-1/hsp32 and hsp70, in isolated rat hepatocytes. Both genes were induced by cellular glutathione depletion. This induction could be inhibited by BAPTA-AM. Culturing in a calcium-free medium prevented the induction of hsp70 gene expression after glutathione depletion without affecting heme oxygenase-1 gene expression. Thapsigargin increased the gene expression of heme oxygenase-1 but not that of hsp70. Thapsigargin-induced heme oxygenase-1 induction was completely inhibited by BAPTA-AM. Incubation with the Ca2+-ionophore A23187 augmented heme oxygenase-1 (two-fold) and hsp70 (5.2-fold) mRNA levels. Our data suggests a significant role of Ca2+-dependent pathways in the induction of the two stress genes. An increase in the cytoplasmic Ca2+ activity seems to play a key role in the cascade of signaling leading to the induction of the two genes. However, the source of Ca2+ that fluxes into the cytoplasm seems to be different. Our data provides evidence for a compartmentalization of calcium fluxes, i.e. the Ca2+ flux from intracellular stores (e.g. the endoplasmic reticulum) plays a major role in the induction of heme oxygenase-1. By contrast, Ca2+ flux from the extracellular medium seems to be a mechanism initiating the cellular signaling cascade leading to hsp70 gene induction.

Słowa kluczowe

EN

heme oxygenase-1; stress response gene; gene expression; hepatocyte; cell protection; heat shock protein; rat; calcium signalling

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2007
• Tom: 12
• Numer: 1
• Opis fizyczny: p.25-38,fig.,ref.

Twórcy

autor

Silomon M

• University of Saarland, 66421 Homburg/Saar, Germany

autor

Bauer I.

autor

Bauer M.

autor

Nolting J.

autor

Paxian M.

autor

Rensing H.

Bibliografia

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