PL
Technikę AFLP (Amplified Fragment Length Polymorphism) zastosowano do identyfikacji gatunku і do oceny wewnątrzgatunkowej zmienności szczepów B. pertussis, В. parapertussis oraz В. bronchiseptica. Wykazano przydatność metody AFLP do określania gatunkowej przynależności szczepów B. pertussis, В. parapertussis і В. bronchiseptica oraz wewnątrzgatunkowego różnicowania szczepów B. pertussis i B. bronchiseptica.
EN
In this study, Amplified Fragment Length Polymorphism (AFLP) method was used to track differences among human and animal isolates of B. pertussis, B. parapertussis and B. bronchiseptica species. One hundred and sixty representative strains of these species orginated from international and Polish bacterial collections were genotyped according to AFLP involving EcoRl/Msel and Spel/Apal restriction/ligation/amplification procedures. This study has confirmed high potential AFLP Spel/Apal procedure for intra-species differentiation of B. pertussis and B. bronchiseptica strains. Both AFLP EcoRl/Msel and Spel/Apal procedures have been found to be useful for species-specific classification in case of B. pertussis strains. In case of B. bronchiseptica or B. parapertussis species-specific classification, Spel/Apal procedure has been found more precise than EcoRl/Msel one.