Interaction of HIV Tat model peptides with tRNA and 5S rRNA

• Autorzy: Giel-Pietraszuk M. , Barciszewska M.Z. , Mucha P. , Rekowski P. , Kupryszewski G. , Barciszewski J.
• Języki publikacji: EN

Abstrakty

EN

New data are presented on the interaction of model synthetic peptides con­taining an arginine-rich region of human immunodeficiency virus (HIV-Tat), with native RNA molecules: tRNA Phe of Saccharomyces cerevisiae and 5S rRNA from Lupinas luteus. Both RNA species form complexes with the Tatl (GRKKRRQRRRA) and Tat2 (GRKKRRQRRRAPQDSQTHQASLSKQPA) pep- tides, as shown by electrophoretic gel shift and RNase footprint assays, and CD measurements. The nucleotide sequence UGGG located in the dihydrouridine loop of tRNA Phe as well as in the loop D of 5S rRNA is specifically protected against RNases. Our data indicate direct interactions of guanine of RNA moie­ties with argininc residues. These interactions seem similar to those observed in DNA-protein complexes, but different from those previously observed in the TAR RNA-Tat complexes.

Słowa kluczowe

EN

tRNA; HIV virus; interaction; protein-nucleic acid interaction; DNA-protein complex; nucleotide sequence; polypeptide; arginine; Saccharomyces cerevisiae; rRNA; Lupinus luteus; man; synthesis; RNA molecule; RNA isolate

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1997
• Tom: 44
• Numer: 3
• Opis fizyczny: p.591-600,fig.

Twórcy

autor

Giel-Pietraszuk M.

• Polish Academy of Sciences, Z.Noskowskiego 12-14, 61-704 Poznan, Poland

autor

Barciszewska M.Z.

autor

Mucha P.

autor

Rekowski P.

autor

Kupryszewski G.

autor

Barciszewski J.

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